Animal experiments are essential to the study of animal nutrition. Because of the large variations among individual animals and ethical and economic constraints, experimental designs and statistical analyses are particularly important in animal experiments. To increase the scientific validity of the results and maximize the knowledge gained from animal experiments, each experiment should be appropriately designed, and the observations need to be correctly analyzed and transparently reported. There are many experimental designs and statistical methods. This editorial does not aim to review and present particular experimental designs and statistical methods. Instead, we discuss some essential elements when designing an animal experiment and conducting statistical analyses in animal nutritional studies and provide guidelines for submitting a manuscript to the Asian-Australasian Journal of Animal Sciences for consideration for publication.
ObjectiveReducing roughage feeding without negatively affecting rumen health is of interest in ruminant nutrition. We investigated the effects of roughage sources and concentrate types on growth performance, ruminal fermentation, and blood metabolite levels in growing cattle.MethodsIn this 24-week trial, 24 Hanwoo cattle (224±24.7 kg) were fed similar nitrous and energy levels of total mixed ration formulated using two kinds of roughage (timothy hay and ryegrass straw) and two types of concentrate mixes (high starch [HS] and high fiber [HF]). The treatments were arranged in a 2×2 factorial, consisting of 32% timothy–68% HS, 24% timothy–76% HF, 24% ryegrass–76% HS, and 17% ryegrass–83% HF. Daily feed intakes were measured. Every four weeks, blood were sampled, and body weight was measured before morning feeding. Every eight weeks, rumen fluid was collected using a stomach tube over five consecutive days.ResultsThe mean dry matter intake (7.33 kg) and average daily gain (1,033 g) did not differ among treatments. However, significant interactions between roughage source and concentrate type were observed for the rumen and blood parameters (p<0.05). Total volatile fatty acid concentration was highest (p<0.05) in timothy–HF-fed calves. With ryegrass as the roughage source, decreasing the roughage inclusion rate increased the molar proportion of propionate and decreased the acetate-to-propionate ratio; the opposite was observed with timothy as the roughage source. Similarly, the effects of concentrate types on plasma total protein, alanine transaminase, Ca, inorganic P, total cholesterol, triglycerides, and creatinine concentrations differed with roughage source (p<0.05).ConclusionDecreasing the dietary roughage inclusion rate by replacing forage neutral detergent fiber with that from non-roughage fiber source might be a feasible feeding practice in growing cattle. A combination of low-quality roughage with a high fiber concentrate might be economically beneficial.
The hand-held laser methane detector (LMD) technique has been suggested as an alternative method for measuring methane (CH4) emissions from enteric fermentation of ruminants in the field. This study aimed to establish a standard procedure for using LMD to assess CH4 production in cattle and evaluate the efficacy of the protocol to detect differences in CH4 emissions from cattle fed with diets of different forage-to-concentrate (FC) ratios. Experiment 1 was conducted with four Hanwoo steers (584 ± 57.4 kg body weight (BW)) individually housed in metabolic cages. The LMD was installed on a tripod aimed at the animal's nostril, and the CH4 concentration in the exhaled gas was measured for 6 min every hour for two consecutive days. For the data processing, the CH4 concentration peaks were identified by the automatic multi-scale peak detection algorithm. The peaks were then separated into those from respiration and eructation by fitting combinations of two of the four distribution functions (normal, log-normal, gamma, and Weibull) using the mixdist R package. In addition, the most appropriate time and number of consecutive measurements to represent the daily average CH4 concentration were determined. In Experiment 2, 30 Hanwoo growing steers (343 ± 24.6 kg BW), blocked by body weight, were randomly divided into three groups. Three different diets were provided to each group: high FC ratio (35:65) with low energy concentrate (HFC-LEC), high FC ratio with high energy concentrate (HFC-HEC), and low FC ratio (25:75) with high energy concentrate (LFC-HEC). After ten days of feeding the diets, the CH4 concentrations for all steers were measured and analyzed in duplicate according to the protocol established in Experiment 1. In Experiment 1, the mean correlation coefficient between the CH4 concentration from respiration and eructation was highest when a combination of two normal distributions was assumed (r = 0.79). The most appropriate measurement times were four times, two hours and one hour before, and one hour and two hours after morning feeding. Compared with LFC-HEC, HFC-LEC showed 49% and 57% higher CH4 concentrations in exhaled gas from respiration and eructation, respectively (P < 0.01). In conclusion, the LMD method can be applied to evaluate differences in CH4 emissions in cattle using the protocol established in this study.
There has recently been an increase in interest in structural health monitoring (SHM) using wireless sensor networks. For SHM, in particular, it is important to accurately and efficiently measure the dynamic acceleration response using wireless sensor networks in real-time. For the purpose, a CAFB (cochlea-inspired artificial filter bank) has been developed in a previous study, which is a dynamic data compression technology. Since the developed CAFB can select and compress only the interested range of frequency signals from an entire response of a structure, it efficiently provides a real-time dynamic response based on wireless networking. CAFB of the previous study is optimized to selectively acquire low-frequency signals of sub-10 Hz, which is required for SHM of long and large-scale structures. According to the CAFB's optimization using an El-Centro seismic waveform, six band-pass filters, 1.0 Hz interval, and 0.6 Hz bandwidth have been adapted. This article is to evaluate dynamic acceleration response performance of civil structures using the CAFB developed in the previous study. To achieve the purpose, the optimally-designed CAFB was embedded in an intelligent data acquisition (IDAQ) system. To evaluate the performance of the IDAQ system with the embedded CAFB, the real-time dynamic response was investigated for a model cable-stayed bridge, measured by a wire-measuring system and the CAFB-based IDAQ system simultaneously. The results show excellent agreement between the compressed dynamic response acquired by the CAFB-based IDAQ system and that acquired by the wire measuring system. In addition, the measurement from the CAFB-based IDAQ system revealed the modal information of the model bridge. The developed CAFB can determine and reconstruct the entire dynamic response from compression with modal information only; its efficient operation illustrates its potential to be utilized in real-time structural health monitoring.
The objective of this study was to evaluate the associative effects of rice straw with timothy hay and corn grain. Using an automated gas production system, in vitro ruminal fermentation was studied for six substrates: 100% rice straw, 100% timothy hay, 100% corn grain, 50% rice straw and 50% timothy hay, 50% rice straw and 50% corn grain, and 50% rice straw, 25% timothy hay, and 25% corn grain. Incubation was performed in three batches with different rumen fluids to assess the in vitro ruminal gas production kinetics and rumen parameters (pH, NH3-N, volatile fatty acid (VFA), and true dry matter digestibility (TDMD)). The associated effects were tested by comparing the observed values of the composited feeds and the weighted means of individual feeds. There was a significant increase in NH3-N when rice straw was fermented with timothy hay, corn grain, or both (p < 0.05). TDMD increased when corn grain was co-fermented, and the total gas and VFA production increased when all three feeds were co-fermented. We conclude that the feed value of rice straw increases when fed to animals along with timothy hay and corn grain.
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