Rebon Shrimp Paste (RSP) in Indonesia uses different percentages of salt addition, ranging from 2 to 20% or not at all. This study aims to determine the influence of different salt concentration (5%, 10%, 15% and without salt) on the quality of RSP organoleptic, microbiological and chemical. This research was conducted in Munjung Agung, Tegal and Cirebon Fisheries Product Quality Testing and Application Laboratory. The results showed that the addition of different salt concentration (5%, 10%,15% and without salt) affected the quality of organoleptics, microbiology, and chemistry. Organoleptic quality with salt concentration of 5% and 10% favored panelists with an average value of 6.8 (not yet meeting Indonesian National Standards). The highest water content value is found in RSP that are not added salt (40,19%-43,22%) and lowest at 15% salt concentration (31,12%-34,82%) in accordance with the SNI. E.Coli and Salmonella growth was negative in all four samples and didn’t different materially, according to SNI standard 2716.1:2009. The best increase in salt concentration in the study was 5%, with an average organoleptic value of 6.8 in the coliform contamination sample A2 APM/g < 3, negative E.Coli and Salmonela with a water content value of 39.15.
Karamunting (Rhodomyrtus tomentosa) merupakan salah satu tanaman yang secara tradisional dimanfaatkan sebagai tanaman obat, seperti mengobati sakit kepala. Tujuan penelitian ini untuk menguji efek analgetik ekstrak etanol daun karamunting diperoleh dari desa Batunabolon,Toba Samosir, Sumatera Utara. Ekstrak dibuat dengan cara maserasi serbuk daun karamunting dalam etanol 70%. uji analgetik ekstrak daun karamunting dilakukan dengan metode induksi kimia pada 25 ekor mencit jantan yang dibagi dalam 5 kelompok perlakuan yaitu kontrol negatif menggunakan CMC 0,5%, kontrol positif diberikan asetosal dengan dosis 1,3 mg/20 g BB mencit dan kelompok perlakuan ekstrak daun karamunting dengan variasi dosis sebesar 200, 400, dan 800 mg/20 kg BB mencit. Hasil penelitian menunjukan bahwa ektrak etanol daun karamunting pada mencit dengan dosis 200, 400, dan 800 mg/20 kg BB mencit mempunyai daya analgetik sebesar 46,87 %, 54,54 %, dan 64,20 %.
Chicken is important livestock in the world that is kept for egg and meat productions. This study was aimed to perform a bioinformatics analysis in the whole genome mtDNA (16,979 bp) of many chicken breeds. Total 84 whole-genome mtDNA sequences were used in this study and obtained from the reference sequence (GenBank). Four molecular packages of BioEdit, MEGA-X, DNAsp, and Arlequin were used in this study to analyze the observed sequences. Therefore, the whole genome mtDNA sequence of birds in this study was originated from Asia, the USA, and New Guinea. Research showed that a total of 445 mutation sites and 81 haplotypes were obtained in this study. Thus, the nucleotide diversity in the observed sequences included of high (0.99). In addition, a total of 10 clusters were observed according to the phylogenetic analysis with the UPGMA method. In conclusion, a close genetic relationship was observed among chickens of Asia based on the median-joining network. Meanwhile, the geographical factors were contributed about 9.12% in a sequence variation.
Pakan merupakan biaya terbesar dalam produksi peternakan. Pakan yang mengandung antibiotik sudah dilarang penggunaanya karena menimbulkan residu pada ternak sehingga dapat mengganggu kesehatan manusia. Solusi untuk mengganti penggunaan antibiotik adalah dengan penggunaan prebiotik. Salah satu sumber prebiotik adalah ubi jalar. Potensi ubi jalar sebagai sumber prebiotik karena adanya senyawa rafinosa dan meltotriosa. Oligosakarida berupa rafinosa pada ubi jalar merupakan sumber makanan bagi probiotik, karena di dalam usus rafinosa tidak diserap sehingga mikroba berperan dalam mencerna gugus gula rafinosa. Selain itu, produksi ubi jalar juga sangat melimpah di Indonesia. Penelitian ini bertujuan untuk menguji efektivitas pemberian prebiotik ubi jalar merah, putih dan ungu dengan menggunakan cairan rumen untuk melihat kecernaan bahan kering, bahan organik, VFA total dan total bakteri secara in vitro. Metode penelitian dilakukan dengan cara in vitro dan koloni bakteri dihitung dengan metode pencacahan koloni bakteri hidup dengan 4 perlakuan dan 3 kali ulangan. Berdasarkan hasil penelitian ini disimpulkan bahwa ubi putih merupakan sumber prebiotik yang paling baik digunakan, karena pada ubi putih jumlah koloni bakteri probiotik yang tumbuh lebih tinggi dibandingkan ubi merah, ungu, dan ransum kontrol.
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