Diare merupakan penyakit yang umum dijumpai, dapat menyerang anak-anak dan orang dewasa. Diare ditandai dengan terjadinya peningkatan frekuensi defekasi yang disertai dengan feses yang cair. Penggunaan obat baik sintetis maupun alami telah diketahui dapat menanggulangi diare yang terjadi, salah satu tanaman sebagai obat alami yang digunakan masyarakat secara empiris adalah daun leunca (Solanum americanum Miller) namun belum ada bukti ilmiah penggunaan empiris tersebut. Penggunaan di masyarakat berupa rebusan daun leunca mendorong peneliti melakukan pengujian aktivitas antidiare infusa daun leunca (Solanum AmericanumMiller.) pada mencit jantan galur Swiss Webster dengan metode proteksi terhadap oleum ricini dan transit intestinal. Infusa daun leunca pada dosis 0,0325, 0,065, dan 0,13 g/Kg BB memiliki aktivitas antidiare dengan menurunkan bobot feses, dan frekuensi defekasi serta meningkatkan konsistensi feses, disertai kecenderungan dalam penurunan gerak peristaltik usus pada dosis 0,13 g/Kg BB berbeda bermakna terhadap kontrol positif (p≤0,05).
In this study, antioxidant activity and antocyanin derivatives study of extract of red katuk (Euphorbia cotinifolia L.) leaves was done. Antioxidant activity was done by DPPH (2,2-diphenyl 1-picrylhydrazyl) method using ultraviolet-visible spectrophotometer and thin layer chromatography. The result showed that methanol extract had antioxidant activity with IC50 value 33.13 μg/mL. Vitamin C as comparative substance had IC50 value was 5.24 μg/mL. From the acidified methanol extract, two anthocyanin derivatives have been isolated by preparative paper chromatography and characterized by ultraviolet visible spectrophotometry, which were supposed to be peonidin and cyanidin.Keywords: Euphorbia cotinifolia L, antioxidant, antocyaninAbstrakPada penelitian ini dilakukan pengujian mengenai aktifitas antioksidan dan turunan antosianin dari ekstrak daun katuk (merah Euphorbia cotinifolia L.). Aktifitas antioksidan dilakukan dengan metode DPPH (2,2-diphenyl 1-picrylhydrazyl) menggunakan spektrofotometer UV-Vis dan kromatografi lapis tipis (KLT). Hasil penellitian menunjukkan bahwa ekstrak metanol memiliki aktifitas antioksidan dengan nilai IC50 33,13 μg/mL. Vitamin C sebagai senyawa pembanding memiliki nilai IC50 5,24 μg/mL. Dari ekstrak metanol dalam suasana asam, terdapat 2 turunan antosianin yang berhasil diisolasi menggunakan kromatografi kertas preparatif dan dikarakterisasi dengan spektrofotometer UV-Vis, dimana kedua isolate tersebut diketahui sebagai peonidin dan sianidin.Kata kunci : Euphorbia cotinifolia L, antioksidan, antosianin
<p>Obesity prevalence has increased in recent years and has caused serious health problems. This research was carried out to obtain alternative antiobesity therapy with more minimal side effects. Antiobesity activity of rose apple (Syzygium jambos (L.) Alston) leaves on female Wistar rats induced by high carbohydrate food for 45 days and subcutaneously injection of MSG 2 g/kgbw. Extraction was carried out using maceration method 96% ethanol. The test parameters observed were body weight, food intake, stool consistency and weight, liver and abdominal fat tissue weight. The results showed that high carbohydrate food and monosodium glutamate could induce obesity. Ethanol extract of rose apple leaves at doses of 25, 50 and 100 mg/kgbw body weight had antiobesity activity by inhibiting body weight gain significantly compased to positive control group (p<0.05). The highest antiobesity effect was shown by the ethanol extract of rose apple leaves at a doses of 50 mg/kgbw with % inhibition of body weight gain of 169.3% to positive control group. Ethanol extract of rose apple leaves may reduce appetite, but didn’t have laxative effect and couldn’t reduce fat deposits in the liver and abdominal fat tissue.</p>
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