Microalgae protein hydrolysates (MPH) were obtained by enzymatic hydrolysis of defatted microalgae meal using neutral protease. The protein recovery, degree of hydrolysis, and the antioxidant activities of the hydrolysates were investigated. The results demonstrated that hydrolysates prepared by neutral protease at 50 °C for 4 h exhibited the strongest antioxidant activity. Under these conditions, the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and the reducing power of the hydrolysates were 68.3%, 50.8% and 1.303, respectively.

To assess the efficacy of the combination of sonographic morphology score (SMS) with CA125 and HE4 for detecting recurrent pelvic ovarian carcinoma (OC). Data of 58 OC patients treated in our hospital between 2014 and 2016 were analyzed. After cytoreductive surgery and routine chemotherapy, all patients were followed up by transvaginal ultrasound examination (SMS for pelvic masses based on volume and structure scores) and tumor marker (serum CA125 and HE4) detection. Clinical diagnosis of recurrent OC was based on physical examination, magnetic resonance imaging, and punctured pathology for pelvic masses. Receiver operating characteristic (ROC) curves of SMS and the tumor markers were generated, and areas under the curve (AUC) values were assessed. There were 26 patients with tumor recurrence and 32 cases with no recurrence. Magnetic resonance imaging had 100% sensitivity and specificity. The areas under the ROC curves of SMS, CA125, HE4, and SMS-CA125-HE4 were 0.816, 0.825, 0.737, and 0.903, respectively. There was no significant difference in AUC values between SMS and CA125 or HE4. There were significant differences in AUC values between SMS-CA125-HE4 and SMS (Z = 2.48, P = 0.042), CA125 (Z = 2.38, P = 0.046), and HE4 (Z = 6.48, P = 0.016), respectively. With a cutoff value of SMS, 5; CA125, 35 U/mL; HE4, 105 pmol/L, the sensitivity, specificity, positive prognostic value, and negative prognostic value of SMS-CA125-HE4 for recurrent OC assessment were 0.9231, 0.8438, 0.8276, and 0.931, respectively. SMS-CA125-HE4 was correlated with recurrent OC (χ2 = 30.7428, P < 0.0001). Ultrasound combined with tumor markers may improve the diagnostic efficiency of recurrent OC.

During the processing of the large yellow croaker, the further utilization of the by-products (e.g., the liver) generated in the processing process can provide opportunities for value addition and increase profitability, promoting the sustainable use of raw materials. Therefore, we have studied the detailed nutritional composition of the large yellow croaker liver (LYCL), which ultimately helps in the development of nutritional and functional products. The results showed that the LYCL has a lower moisture content (45.71%) than fish meat, 8.25% protein, and 37.97% fats. In addition, LYCL contained the full range of amino acids. Unsaturated fatty acids accounted for 62.63%, of which EPA and DHA account for 9.42%. It was rich in phospholipids, vitamins and trace elements. Heavy metal elements such as Hg, As, Cd were lower than the heavy metal limit standards for fish in China. Aldehydes, ketones and alcohols in the liver have the greatest impact on odor.

In order to evaluate the relationship between of endogenous enzymes and physiochemical indicators during the processing of dry-salted Decapterus maruadsi produced by the traditional method. The evolution of the endogenous enzymes (cathepsin B, cathepsin L, cathepsin H, acid lipase, neutral lipase, and phospholipase) and physical and chemical parameters (moisture content, salt content and pH) of dry-salted Decapterus maruadsi during processing were investigated. Principal component analysis and Pearson correlation coefficient analysis were used to explore the main factors influencing the endogenous enzyme activity and the interactions between endogenous enzymes. The following results were observed during the processing of dry-salted Decapterus maruadsi: the salt content could stimulate cathepsin (L and B) activity and inhibit lipase activity; a lower moisture content could inhibit lipase activity, while the inhibitory effect on cathepsin was inconspicuous; pH had a minor influence on the endogenous enzyme activities; cathepsin B and L could partially inhibit lipase activity.Cambios detectados en las enzimas endógenas y los indicadores fisicoquímicos al producir Decapterus maruadsi salado en seco RESUMEN El presente estudio se planteó evaluar la relación existente entre enzimas endógenas e indicadores fisicoquímicos durante el procesamiento de Decapterus maruadsi salado en seco producido por el método tradicional. Al respecto, durante el procesamiento de Decapterus maruadsi salado en seco se investigaron la evolución de las enzimas endógenas (catepsina B, catepsina L, catepsina H, lipasa ácida, lipasa neutra y fosfolipasa) y los parámetros físicos y químicos (contenido de humedad, contenido de sal y pH). Para explorar los principales factores que influyen en la actividad de la enzima endógena y las interacciones entre las enzimas endógenas, se utilizaron análisis de componentes principales y del coeficiente de correlación de Pearson. Durante el procesamiento de Decapterus maruadsi salado en seco se observaron los siguientes resultados: el contenido de sal puede estimular la actividad de las catepsinas (L y B) e inhibir la actividad de la lipasa; un menor contenido de humedad puede inhibir la actividad de la lipasa, mientras que el efecto inhibitorio sobre las catepsinas es discreto; el pH tiene una influencia menor en las actividades enzimáticas endógenas; las catepsinas B y L pueden inhibir parcialmente la actividad de la lipasa.