We present a strategy to fabricate discrete Au nanorods (AuNRs) into controllable side-by-side (SS) and end-to-end (EE) assemblies through bio-recognition of h-IgG and anti-hIgG Fab. Due to anisotropic properties of AuNRs, bifunctional PEG (SH-PEG-COOH) with low concentration preferentially bound to the ends of AuNRs while high concentration and large amount resulted in binding on the side surfaces and then proteins were covalently conjugated to either surface through EDC/NHS. Thus, controllable SS and EE assembly of AuNRs were obtained through antibody-antigen recognition.

We report a new type of biochemical materialmultifunctional paclitaxel-loaded poly lactide-lecithin (PLA-lecithin) microbubbles which has been developed with a method of ultrasonic double emulsion solvent evaporation (UDES) combined with lyophilization. Bubbles were characterized to be hollow, well dispersed andwith size of 300nm~2um. Paclitaxel loading efficiency could reach up to 12%, and bubbles with different size and different lecithin content showed varied drug release characteristics but all displayed slow-release and ultrasound controlled properties, illustrating the ultrasound responsive drug release effect of the combination of microbubbles and ultrasound.

Drug loaded PLA (or PLGA) mirobubbles that combine properties of ultrasound imaging contrast agents and drug carriers suffer from low encapsulation efficiency and difficulty to destruction with diagnosis ultrasound. In this paper, a new type of multifunctional paclitaxel-loaded poly lactide-lecithin (PLA-lecithin) microbubbles has been developed with a method of ultrasonic double emulsion solvent evaporation (UDES) combined with lyophilization, and single-factor of ultrasonic time was studied to influence bubble size and drug loading efficiency. Bubbles were characterized to be well dispersed, with size of 300nm~2um, and showed increased ultrasound imaging on rabbit liver and heart after intravenous injection.