Introduction: Thyroid hormone is known to affect reproductive biology. Abnormal uterine bleeding is one of the common presentations in gynaecology outpatient departments and thyroid dysfunction is known to affect its progression. This study aims to find the prevalence of thyroid dysfunction in diagnosed cases of abnormal uterine bleeding in patients in a tertiary hospital of eastern Nepal. Methods: A descriptive cross-sectional study was conducted in patients diagnosed with abnormal uterine bleeding in a tertiary care hospital of eastern Nepal from April 2019-March 2020 after taking ethical clearance from the Institutional Review Committee. On basis of inclusion and exclusion criteria, 95 cases of abnormal uterine bleeding were included in the study. A blood sample was taken and a thyroid function test was done by chemiluminescence assay on an automated analyzer. Convenient sampling method was used for sample collection. Statistical analysis was done using Statistical Package for the Social Sciences version 16. Point estimate at 95% Confidence Interval was calculated along with frequency and proportion for binary data. Results: Out of the total cases of abnormal uterine bleeding, 15 (15.79%) (8.46-23.12 at 95% Confidence Interval) had thyroid dysfunction. Among total cases, 80 (84.21%) were euthyroid. The mean age of the patients was 33±8 years. Among thyroid dysfunction, 9 (60.0%) were hypothyroid, 4 (26.66 %) were subclinical hypothyroid, and 2 (13.33 %) were hyperthyroid. Conclusions: Thyroid dysfunction was common among patients with abnormal uterine bleeding, with hypothyroidism being the most common type.

Introduction: Alcoholic liver disease is a serious health problem related to an unhealthy lifestyle. The three most widely recognized forms of alcoholic liver disease are alcoholic fatty liver, acute alcoholic hepatitis, and alcoholic cirrhosis. The main aim of our study is to find out the prevalence of alcoholic liver disease in tertiary care center. Methods: A descriptive cross-sectional study was conducted among inpatient cases admitted in the medicine department of tertiary care center from 1st June 2018 to 31st May 2019. Ethical approval was taken for the study. Convenience sampling method was used. All the biochemical parameters were expressed as mean±standard deviation for each group and point estimate at 95% Confidence Interval was calculated along with frequency and proportion for binary data. Results: Prevalence of alcoholic liver disease is 50 (50%) at a 95% Confidence Interval (40.2%-59.8%) and non-alcoholic fatty liver disease is also the same. The mean age of alcoholic liver disease was 59±12 years where as the mean age for non-alcoholic fatty liver disease was 46±18 years. Out of fifty patients of alcoholic liver disease, majority 48 (96%) of the cases were males which suggests that the prevalence of alcoholic liver disease is very common in males. Similarly, for non-alcoholic fatty liver disease, prevalence was 34 (68%) showing higher prevalence than that of females. Conclusions: Prevalence of alcoholic liver disease is low compared to previous studies done in the similar settings. Monitoring these biochemical parameters in alcoholic liver disease at early stage could guide in planning the protocol for the initial treatment.

Introduction: Pre-eclampsia, one of the most common medical complication in pregnancy results in considerable maternal and fetal morbidity and mortality. Endothelial dysfunction is a central feature of pre-eclampsia. Elevated serum uric acid level may serve as a marker for early diagnosis of the disease as well as a surrogate for clinical severity of the condition. High serum values of triglycerides, total cholesterol, low- density lipoprotein and and low levels of high density lipoprotein are all significantly related to risk developing preeclampsia.  Objective: To find out the association between serum uric acid levels and lipid profile in pre-eclamptic women and compare it with the normal pregnant women.  Methodology: A case control study was conducted among 180 pregnant women (90 cases of pre-eclamptic women and 90 cases of age matched normal pregnant women) between 21-35 years who were admitted in the Gynaecology and Obstetrics ward of Nobel Medical College and Teaching Hospital, Biratnagar. Blood pressure was measured. Serum from all the patients were analyzed for the following biochemical parameters: serum uric acid, triglyceride, total cholesterol, LDL-C and HDL- C. The data were collected and entered in MS-Excel and analyzed using Statistical Package for Social Sciences (SPSS) ver. 16 software. Results: Mean serum uric acid of pre-eclamptic women was higher than those of normal pregnant women  which was found to be statistically significant with p value <0.001. Among the lipid parameters, serum triglyceride and total cholesterol was significantly higher in pre-eclamptic women whereas high density lipoprotein was significantly lower in pre-eclamptic. Also there was a positive co-relation between triglyceride and blood pressure and a negative co-relation between HDL-C in preeclamptic women. Conclusion: Development of simple and inexpensive methods to predict and prevent pre-eclampsia in early stage is very important. Thus, our study concludes the utility of measurement of serum uric acid and lipid profile for screening patients at risk of developing pre-eclampsia.

Background: Diabetes mellitus (DM) is a non-communicable metabolic disease resulting from either insulin deficiency or insulin resistance. Liver enzymes (ALT and AST) are the well-known markers of hepatocellular health while GGT also shows biliary tract function. Increased activities of liver enzymes are indicators of hepatocellular injury, are associated with insulin resistance and Type 2 Diabetes Mellitus. Aims and Objective: To study the status of Liver Enzymes in type 2 diabetes patients residing in the eastern part of Nepal. Materials and Methods: This was a descriptive cross-sectional study conducted at the Department of Clinical Biochemistry, Nobel Medical College Teaching Hospital, Biratnagar, Nepal dated from 27th Dec 2019 to 27th Dec 2020. The blood sample was taken from the patients coming to the outpatient department at Diabetic and Endocrinology Clinic for a regular check-up and follow-up and those willing to participate in research. All the data collected was entered in Microsoft Excel and Statistical Package for Social Service (SPSS) version 16. p < 0.05 was considered to be statistically significant. Results: A total of 375 subjects (255 DM and 120 healthy) were included. The age of the diabetic subjects ranges from 27-87 years with a mean of 56.91 ± 11.00 years while age of healthy subjects’ ranged from 31-86 years with the mean of 53.38 ± 13.28. Among the T2DM subjects, 11.76% (30/255) had raised AST, 17.25% (44/255) had raised ALT, 12.94% (33/255) had raised ALP, and 19.60% (50/255) had raised GGT. The level of liver enzymes (AST, p = 0.005, ALT, p = 0.007, ALP, p = 0.000 and GGT, p = 0.000) were showed statistically significant. Conclusion: This study concludes that liver enzyme activity was higher in T2DM subjects than individuals who do not have T2DM. In addition, Liver parameters were significantly correlated with diabetes mellitus in our study population; hence, timely diagnosis and management of the abnormal liver parameters may help to minimize liver-related morbidity and mortality in the diabetic population.

Background: Low density lipoproteinforms a basis of decision making in treatment of hypercholesterolemic patients and primary target of intervention. Its cost effective and accurate measurementis a need for every clinical laboratories and different calculation methods has been adopted as a replacement to direct assays. This study aims to evaluate the Martin’s formula and Friedewald’s formula in a sample of Nepalese population compared against direct homogenous assay. Materials and Methods: This is a cross-sectional study conducted in Department of Biochemistry from Feb 2020 to January 2021. Serum samples of the participants were analysed for total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein. Martin’s and Friedewald’s formula were applied to get calculated value of low density lipoprotein from both methods. Passing and Bablok regression analysis was used for methods comparison. Results: The mean age of participants was 54.2 ± 8.9 years. Passing-Bablok regression analysis showed Friedewald’s formula performed better than Martin’s formula as per systematic and proportional bias when compared with directassay. However at lowerserum low density lipoprotein level, underestimation of low density lipoprotein compared to direct assay was more common in Friedewald’s formula. At high triglyceride level more percentage error of difference of mean from direct assay was found for Friedewald’s formula. Conclusion: When compared to direct assay, Friedewald’s formula was found to be in better agreement than Martin’s formula. Martin’s formula had advantage over Friedewald’s formula at lower serum low density lipoprotein level and higher triglyceride level where Friedewald’s formula mostly underestimated low density lipoprotein.