No abstract

This study was carried out, during 2011, to determine the prevalence of Eimeria spp. and Cryptosporidium spp. infecting cattle in Kafr El-Sheikh province. Faecal samples (n=698) were examined with flotation technique for Eimeria and modified Ziehl-Neelsen staining technique for Cryptosporidium spp. Oocysts. Eimeria spp. were in 28.94% and Cryptosporidium spp. in 34.1 %. Eleven Eimeria spp.

The present study was conducted to elucidate the prevalence of Cryptosporidium bovis in suckling and weaned cattle calves (Bubalus bubalis) from different governorates in northern, middle, and southern Egypt, such as Behera, Menofia, Qaliubiya, Assiut, and Sohag; result revealed that from the overall examined fecal samples (n = 825), the overall prevalence was 7.27%, the highest significant infection rate was in young suckling calves less than one month (8.2%), and seasonally, winter season has the highest significant level (11.24%), but sex and locality were of no significant effect on the prevalence of infection in this study. Gene sequencing and phylogenetic analysis of the 18SSU-rRNA gene of the local bovine isolate were performed, and it was found that C. bovis genotype was highly similar to human isolate, which provoke the zoonotic transmission of bovine isolate to humans and identified as a potential source for human cryptosporidiosis infection in Egypt.

Background, Methods: To elucidate the prevalence and molecular characterization of Giardia infection in dogs, a cross-sectional study was performed on stray and housed dogs from different localities of Egypt. Results: A total of 986 fecal specimens were collected from dogs. The overall infection rate was 8.5%. The diarrheic dogs revealed higher prevalence of 14.43% than asymptomatic animals. The infection was more prevalent in younger dogs ( < 6 months) (9.5%) compared to older animals. Higher prevalence of infection was observed during the cold winter months (11.24%). The community owned dogs (stray dogs in the street) showed (11.75%) higher prevalence rate than pet dogs in the household environment (5.59%). Moreover, it was found that dogs fed on undercooked meat, and offal's were showing higher prevalence of giardiasis than dogs fed on canned meat. On the other hand, dogs subjected to regular grooming and good hygienic practices had lesser prevalence rate of the infection compared to unclean neglected dogs. Conclusions: The sequencing and phylogenetic analysis of the amplicons of 18SrRNA gene of G. duodenalis revealed that; they were closer to assemblage D necessitating urgent attention due to their zoonotic importance.

M esostephanus appendiculatus (Family: Cyathocotylidae) is one of the unique trematodes that complete their cycles in human and animal intestines in many countries of the world. The main source of its transmission is eating raw or undercooked infected fish muscle. Earliest analyses of genes to different parasites supported the analysis of helminthes either biological or morphological. This paper detected M. appendiculatus sequence with GenBank accession number gb (KY026782). Comparison of M. appendiculatus with other helminthes using BioEdit 7 and MEGA7 program shows some similarity in different points along its sequence. The phylogenetic analysis clarifies that it was closely related to both trematodes (Clinostomum complanatum and Echinochasmus japonicus) and some cestodes of fish origin such as Polyonchobothrium polypteri, Bothriocephalus sp., and Haplobothrium globuliforme. The obtained results provide a good source for genome analysis of M. appendiculatus in relation to other Platyhelminthes.