Résumé On décrit les réactions d'un sérum qui agglutine la majorité d'échantillons d'érythrocytes humains. Le donneur de ce sérum est un noir, M. Luke P., qui souffrait de tumeur lymphomateuse; il n'avait jamais été préalablement transfusé. Il y a trois degrés de réactions: la plupart des échantillons sont Luke(1) ou Luke(w); les réactions Luke(‐) sont présentes chez les erythrocytes des personnes de phénotype rare «p» ou «Pk», et, ceci parmi le 2% des caucasiens pris au hasard. Les réactions Luke(w) et Luke(‐) sont plus fréquentes avec les erythrocytes P2 qu'avec les erythrocytes P1 et, également, plus fréquentes avec les erythrocytes A1 et A1B qu'avec les erythrocytes O, A2, A2B et B. De toute manière, le rapport qui existe entre les erythrocytes P et ABO demeure obscur. L'agglutinine se trouvant dans le sérum Luke est particulièrement difficile à absorber. Les réactions Luke sont certainement sous le contróle génétique et le phénotype Luke(—) est probablement de caractère récessif. Zusammenfassung Es werden die Reaktionen eines Serums beschrieben, welches die überwiegende Mehrzahl der menschlichen Erythrozyten agglutiniert. Der Serumspender war ein Neger namens Luke P., der an lymphomatösen Tumoren litt; er hatte nie Blut‐transfusionen erhalten. Es wurden drei Reaktionstypen gefunden: die meisten Blutproben sind Luke(+) oder Luke(w); Luke(—)‐Reaktionen wurden lediglich bei Blutproben der seltenen Phänotypen p oder Pk sowie bei ca. 2% der Blutproben der weißen Durchschnittsbcvölkerung beobachtet. Luke(w)‐ und Luke(—)‐Reaktionen waren hàufiger bei P2‐als bei P1–lutproben; sie waren auch hàufiger bei Blutproben der Gruppen A, und A1B als solchen der Gruppen O, A2, A2B und B. Trotz aller Bemühungen gelang es nicht die Beziehung zu den P‐und ABO‐Gruppen zu klären. Das im Luke‐Serum enthaltene Agglutinin läßt sich durch Absorption kaum entfernen. Die Luke‐Reaktionen unterliegen ohne Zweifel einer genetischen Steuerung; der Luke(—)‐Phänotyp ist vermutlich ein rezessives Erbmerkmal.

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The anticoagulant factor Protein S circulates in two major forms, free and bound to C4b binding protein (C4BP). We report a monoclonal antibody-based enzyme-linked immunosorbent assay of human Protein S that is sensitive (detection limit 30 ng) and free of notable competition from other vitamin K-dependent proteins. We demonstrate assay sensitivity to Protein S-C4BP complex, as well as to free Protein S by (a) results of immunoaffinity chromatography involving the assay antibody; (b) parallelism between the standard curve and a plasma dilution curve, including dilutions greater than 100-fold; (c) lack of effect of added C4BP on Protein S values; and (d) reactivity in the assay by high-molecular-mass plasma Protein S in gel permeation studies. These latter studies also indicate that uncomplexed Protein S has an apparent molecular mass of approximately 150,000 Da, attributable to either polymerization or conformational considerations. Inter- and intra-assay CVs were 9.8% and 5.1%, respectively, and 90% of Protein S added to plasma was recovered. Reference ranges (mean and SD) for total Protein S in middle-aged normal subjects were 16.6 (1.9) mg/L for men (n = 54) and 16.0 (3.0) mg/L for women (n = 38). In the polyethylene glycol precipitation method, the reference values for free Protein S were 5.4 (1.6) mg/L for men and 4.4 (1.1) mg/L for women.

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A aerum (J.D.) obtained from a donor of type RhlRh,(CCDee.) was determined by the usual atudies to be anti-hr'(c). It was eventually found, however, to give no reaction with an occarional blood sample which was hr'-pitive with all other typing serums. Cells of the latter phenotype (J.U.negative, hr'-positive) rhow a weakened expression of both hr' and hr(f). The characteristic is inherited, is expressed in the heterozygote, and has not yet been found in the hamozygoua form. The J.D. aerum has been designated as anti-Rh26, hence the Rh phenotype identified by it is Rh: w4, w6, -26. Most hr'-positive red blood c e h also pos-IUW Rh26. One donor Jnmesaing Rh26 but not hr' has been detected.SINCE the retraction of cv.2 the Rh factor hr'(c) has not been subtyped and seldom found to be weakly expressed,3 nor has anti-hr' been considered a mixed antibody. A recently discovered serum has, however, revealed a distinct, inherited Rh phenotype, in which the expression of hr' and hr is affected, and which was initially thought to represent a variant of hr'. The SerumA potent anti-hr'(c) was obtained from J.D., a young woman with a history of having had three transfusions in 1958 for an ectopic pregnancy, followed by two stillbirths, in 1960 and 1961, apparently due to crythroblastosis. She is group 0, phenotype Rh,Rh, (CCDee); her husband is group A, phenotype Rh,rh (ccDEe). The J.D. serum had a titer of 1:16 (antiglobulin) against Rhlrh (CcDee) cells, 1 : 32 against rh (cde) cells. It was also active against saline-