Background: Alzheimer's disease is the formaton of amyloid beta (Aβ) plaques and tau tangles in the brain. As Aβ is an important factor in the pathogenesis of Alzheimer's disease, the present research aims to consider the effects of six weeks of high intensity interval training on Aβ1-42 levels in the hippocampus tissue of male rat models of Alzheimer's disease.Methods: Thirty-five rats (3 months old, 222.83 ± 19.60 g) were divided into five groups: Two experimental, two control and sham.Alzheimer's disease, induced by streptozotocin was infused into the rats i.c.v (a 3 m.g/kg dose). Their memory was evaluated by passive avoidance learning method using a shuttle box. The amount of peptide Aβ1-42 was measured by the ELISA method. Comprisions between groups were performed by One-way ANOVA followed by Tukey test. A value of P < 0.05 was considered to be significant. Results:The Aβ1-42 levels in the hippocampus of Alzheimer's control group was significantly higher than Alzheimer's HIIT group (P = 0.036) and it was lower in the health HIIT group than it was in the health control group (P = 0.001). Conclusions:It declare that HIIT training lessen the level of Aβ1-42 in the hippocampus and can be benefit for AD rat model. As a result, the advantages of HIIT training can be used in protection and treatment of AD.