Background: Shukti (Oyster) is a very commonly occurring calcium form. It is rich source of calcium & minerals. As per text it can be converted into two forms which are bhasma (calcinated ash) and pishti (powdered form without agni).These forms may have different rate of absorption. This needs to be studied. Aim: To study Pharmaceutico-analytical study of Muktashukti pishti & Muktashukti bhasma and comparative evaluation of their relative oral bioavailability. Materials and methods: The two formulations will be prepared from shukti (oyster). By triturating with Gulabjala Muktashukti pishti will be prepared and by traditional puta method Muktashukti bhasma will be prepared. The prepared formulations will be assessed for Bhasma Pariksha mentioned in Ayurveda. Organoleptic characters, physicochemical parameters and Particle size distribution analysis, SEM-EDX (Scanning Electron Microscopy, Energy Dispersive X-Ray Analysis), FTIR (Fourier-transform infrared spectroscopy), XRD (X-Ray Diffraction), GCMS (Gas Chromatography Mass Spectrometry) will be evaluated. To assess the relative oral bioavailability of Muktashukti pishti & Muktashukti bhasma study will be conducted in healthy volunteers and will be compared with the standard calcium supplement. The study will be conducted in between two test groups and standard group. Observation and results: The analytical parameters will be assessed and compared in Muktashukti bhasma and Muktashukti pishti. For relative oral bioavailability Blood serum calcium will be assessed in all three groups. By applying unpaired “t” Test, One-way ANOVA the statistical significance can be measured. Conclusion: The pharmaceutical & analytical study of Muktashukti pishti and Muktashukti bhasma will provide the standard parameters and clinical comparative evaluation with standard will generate evidence for better bioavailability.

Shukti is an important component of Sudhavarga, the most recent class in the subject of Rasa Shastra. According to the availability, two forms of Shukti have been stated in Rasa Shastra texts: Jalashukti and Muktashukti. Muktashukti is considered for the study. This study has tried to develop the standard operating process for manufacturing Muktashukti bhasma and Muktashukti pishti. Three batches of MSB & MSP were prepared from the purified Muktashukti. While manufacturing three batches same raw material and manufacturing instruments were used. Before processing for Bhasma or Pishti, the purification process is advocated for Muktashukti. Kanji (Rice gruel) was used for purification as per textual reference. Purified Muktashukti was levigated with Rose water to obtain Pishti. However, for Bhasma preparation, the Muktashukti was triturated with Aloe vera juice, and then heating was done in an Electric muffle furnace. 7 liters of Kanji was taken for 500g of Muktashukti Shodhana by Swedana for 3hrs in Dolayantra, with an average weight rise of 96.24% and a weight loss of 3.76% during the Shodhana method. During MSB preparation, a 10% loss was observed & 90% of Bhasma was obtained. However, in MSP preparation, a 12.14% loss was observed & 87.86% was obtained. This study will give the direction related to the duration of levigation and temperature regulation while Muktashukti Bhasma preparation. For Pishti preparation, the wet grinder was used, which will provide exact rotation and duration to obtain the proper pishti from Muktashukti.

Background: Lohais a metal that is used in many preparations after transforming it into non-metallic form by purification and incineration method uses to treat different kinds of diseases. Mandura is the rusting of iron.Tryushanadyalauha(TL) is one among the Ayurvedicherbo-mineral formulations described in BhaishajyaRatnavalliand as modified dosage form as Tryushanadyamandura(TM).The herbal contents are tryushana (i.epippali( Piper longum Linn ),maricha (Piper nigrum Linn)and shunti (Zingiber Officinale Roscoe), cavya (Piper chabaHunter), bakuchi (Psoralea CorylifoliaLinn),bhang (cannabis sativum Linn),andlavana like saindhava,aubhida,vida and sauvarchala ,andlohabhasma is the main ingredient. Aim: Pharmaceutical and Analytical study of TryushanadyaLauha& modified form as TryushanadyaMandura and comparative evaluation for antidiabetic activity in Wistar rats. Materials and Methods: All herbal drugs will be collected, verified, and primarily authenticated by the Department of Dravyaguna. Lohaand Mandura will be procured from the vendor and authenticated by the Department of Rasashastra and BhaishajyaKalpana, Mahatma Gandhi Ayurved College Hospital Research Centre, Salod(H), Wardha, and they will be prepared as per reference. Organoleptic, bhasmapariksha, Physico-chemical,XRD and FEG-SEM parameters will be evaluated. To assess Tryushanadya Lauha (TL) and Tryushanadya Mandura (TM)antidiabetic action will be conducted in 30 Wistar rats in 5 groups and will be compared. Observation and Results: The study will be assessed Tryushanadya lauha (TL) and TryushanadyaMandura (TM)antidiabetic action in 30 Wistar rats by using one-way ANOVA. Conclusion: Pharmaceutical and Analytical study of TryushanadyaLauha (TL) & modified form as TryushanadyaMandura (TM) will provide the standard parameters.

The present paper describes simple and sensitive, reversed phase high performance liquid chromatography (RP-HPLC) method developed for the determination of tapentadol hydrochloride in bulk and in its tablet dosage form. Chromatographic separation was achieved by using phenomenex C18 column, 250×4.6 mm, 5 μm as stationary phase. Mixture of acetonitrile: water (80:20 V/V) was used as the mobile phase and the pH was adjusted into 7.0 using orthophosphoric acid, at a flow rate of 0.7 mL/ min. Tapentadol standard shows maximum absorption in UV at 272 nm. The method was linear over the concentration range of 5-25 μg/mL. The method was validated statistically and recovery study was performed as per ICH guidelines. The analytical recovery obtained was 98-119%. As per validation data it was found that method is specific, robust and precise within the described concentration range. The described RP-HPLC method was successfully employed for the analysis of a commercial brand of tapentadol hydrochloride tablets.

Background: Arka is commonly found plant in India. It is mostly used for religious purpose in India. In Ayurved, it has in used for various medical preparation. One of such preparation is Arka Lavan. Aim: Pharmaceutico-analytical study of Arka lavan& evaluation for Antimicrobial study Materials: The raw materials Arka patra will be collected from the medicinal plant garden, of Mahatma Gandhi Ayurved College , Hospital & research centre, Salod ( H), wardha. The arka patra will  be authenticated by Dravyaguna department. The another raw material is Saindhava Lavan will be procured from Dattatraya Ayurved Rasashala and will be authenticated by Rasashastra & Bhaishajya kalpana Department Arka lavan will be prepared as per the reference.The prepared Arka lavan will be analyzed. Observation and Results: The Arka patra lavan will be assessed for organoleptic, physicochemical and TLC parameters  Conclusion: The pharmaceutical & analytical study of Arka lava will provide the standard parameters.