Mycorrhizal fungi colonize orchid seeds and induce germination. This so-called symbiotic germination is a critical developmental process in the lifecycle of all orchid species. However, the molecular changes that occur during orchid seed symbiotic germination remain largely unknown. To better understand the molecular mechanism of orchid seed germination, we performed a comparative transcriptomic and proteomic analysis of the Chinese traditional medicinal orchid Dendrobium officinale to explore the change in protein expression at the different developmental stages during asymbiotic and symbiotic germination and identify the key proteins that regulate the symbiotic germination of orchid seeds. Among 2256 identified plant proteins, 308 were differentially expressed across three developmental stages during asymbiotic and symbiotic germination, and 229 were differentially expressed during symbiotic germination compared to asymbiotic development. Of these, 32 proteins were coup-regulated at both the proteomic and transcriptomic levels during symbiotic germination compared to asymbiotic germination. Our results suggest that symbiotic germination of D. officinale seeds shares a common signaling pathway with asymbiotic germination during the early germination stage. However, compared to asymbiotic germination, fungal colonization of orchid seeds appears to induce higher and earlier expression of some key proteins involved in lipid and carbohydrate metabolism and thus improves the efficiency of utilization of stored substances present in the embryo. This study provides new insight into the molecular basis of orchid seed germination.

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This paper introduced construction method for deep pile of Zishui bridge through karst region and the checking results of the pile bearing capacity. Firstly, main structure of Zishui bridge was simply introduced. Secondly, according to the special geological conditions, construction methods for bored piles in water and drilling platform were described. The discussion focused on the problem of boring and grouting in karst foundation and their solutions. Finally, to ensure that the pile capacity can meet the design requirements, load transfer method was used on the part of pile foundation to calculate the bearing capacity. Results showed that pile bearing capacity meet the requirements, and the feasibility and correctness of construction method of Zishui bridge was also verified,which provides references for the design and construction of the same civil engineering.