Nowadays, polymeric nanoparticles have drawn more attention as drug carrier by investigators.In this study, we synthesized high selective imprinted nanoparticle polymer using olanzapine as template. The aim of this study was preparing efficient imprinted polymer nanoparticles from olanzapine as the template for the controlled release of olanzapine as a therapeutic drug for central nervous systems (CNS) disease at different pH values and the solid-phase extraction (SPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC). The morphology of the nanoparticles was determined using scanning electron microscopy (SEM) images. Drug release, binding properties and dynamic light scattering (DLS) of the molecularly imprinted polymers (MIPs) were studied in this investigation. The adsorption isotherm was fitted with Langmuir and Freundlich models. The performance of the MIPs for the controlled release of olanzapine was assessed in two different media (SDS 1% and PBS). Results revealed that the MIPs have potential application in controlled drug release. Moreover, cytotoxicity of the MIP nanoparticles was measured on NIH/ 3 T3 cell line using MTT method.Furthermore, the MIPs were applied to extraction of olanzapine from human blood plasma samples. The limit of detection (LOD) and limit of quantification (LOQ) were evaluated and were 0.18 µg L -1 and 0.39 µg L -1 , respectively. These results collectively illustrate that MIP nanoparticles can be employed as an efficient technique for the extraction of the olanzapine from human plasma.

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Herein, we report a magnetic molecularly imprinted polymers (m-MIPs) using Fe 3 O 4 as a magnetic component, carvedilol as a template molecule for the solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography (HPLC) and for the controlled release of carvedilol at different pH values of 1.0 (simulated gastric fluid), 6.8 (simulated intestinal fluid), and 7.4 (simulated biological fluid). The adsorption kinetics was modeled with the pseudo-first-order and pseudosecond-order kinetics, and the adsorption isotherms were fitted with Langmuir and Freundlich models. The performance of the m-MIPs for the controlled release of carvedilol was assessed and results indicated that the magnetic MIPs also have potential applications in controlled drug release. Furthermore, the m-MIPs were applied to the extraction of carvedilol from human blood plasma samples. Carvedilol can be quantified by this method in the 2-350 lg L 21 concentration range. The limit of detection and limit of quantification in plasma samples are 0.13 and 0.45 lg L 21. The results from HPLC showed good precision (3.5% for 50.0 lg L 21 ) and recoveries (between 85 and 93) using m-MIP from human plasma samples.

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Commercial lipid emulsion of propofol (CLE) has several drawbacks including pain on injection and emulsion instability. In this paper, a novel nanocarrier system is introduced to improve stability and solubility of the poorly soluble anesthetic drug, propofol, for intravenous administration. In this paper, alginate is modified using a facile method in which the carboxylic group of alginate is grafted to octanol. The octanol-grafted alginate (Alg-C8) is then employed to prepare nanoparticles which are subsequently used for encapsulation of propofol. The nanoparticles are analyzed for their pH, osmolarity, particle size, stability, morphology and sleep recovery and the results are compared with CLE as control. It is revealed that nanoparticles have the average particle size of 180 nm ± 1.2 and spherical morphology which is less than CLE while their pH, osmolarity and profile of release of formulated nanoparticles are similar to those of CLE. In addition, the results show good chemical and physical storage stability for the nanoparticles at room temperature for at least 6 months compared to CLE as control. The animal sleep recovery test on rats shows no significant difference in time of unconsciousness and recovery of the righting reflex between nanoparticles and CLE. It is concluded that encapsulated nanoparticles introduced here could be a promising clinical intravenous system for delivery of poorly soluble anesthetic propofol. In addition, this study provides an efficient and facile method for preparing a carrier system for water insoluble drugs.