Tetrahydropentagamavunon-0 (THPGV-0) and Tetrahydropentagamavunon-1 (THPGV-1), are analogs of a curcumin metabolite, tetrahydrocurcumin, and a derivate of Pentagamavunon-0 (PGV-0) and Pentagamavunon-1 (PGV-1), respectively. THPGV-0 and THPGV-1 have been successfully synthesized and are investigated for their anticancer potency. Cytotoxic assays were performed toward several cancer cell lines to determine values of IC50. Assessing cytotoxicity on Vero normal cell line showed the selectivity of those compound. THPGV-1 showed highest cytotoxic activity in lymphoma Raji cells, a suspension cell line, with an IC50 of 180µM. Both THPGV-0 and THPGV-1 showed similar potencies on T47D breast cancer cell line with IC50 values of 250-270µM. Regardless their high selectivity, however, cytotoxic activities of THPGV-0 and THPGV-1 were lower compared to PGV-0 and PGV-1 on HeLa cervical, T47D breast, and WiDr colon cancer cell lines. Further study using different types of cancer cell lines and confirmation of cell viability by another assays and apoptosis detection may give more benefit.

Glagah (Saccharum spontaneum L.) has potential value as a crop species and may also be used in sugarcane breeding programs; however, this germ-plasm has not been extensively used in breeding programs, primarily in relation to improve drought tolerance. The objectives of this experiment were to evaluate the effect of drought stress initiated at vegetative growth stage on morphological (plant height, stalk diameter, green leaf number) and biochemical (leaf proline content and protein pattern) responses of seven glagah accessions (BOT-53, BOT-54, BOT-60, BOT-77, BOT-78, BOT-84, and BOT-88). The plants were propagated from single node stalk segments in polybag in the field under non-stress condition for two months. The two-month-old plants were then subjected to drought stress by withholding watering for eight weeks. Untreated control plants were watered every two days. Results indicated that drought stress reduced plant height, stalk diameter and green leaf number. On the other hand, there was a little difference between drought-stressed and control plants in terms of proline content. The protein pattern showed that drought stress caused a change in gene expression in the form of induction or repression of protein expression. A specific protein with a low range of molecular weight (Rf value about 0.647) showed constitutively expressed in accessio n BOT-53 but drought-inducible expressed in BOT-54.

Abstract Liver disease is one of the leading causes of death globally. Recently, its prevalence and mortality rate continue to increase. It was reported that Indonesia’s hepatitis prevalence was 1.2% in 2013. Indonesia is the world second largest megabiodiversity country and inhabited by 1,068 ethnicities. Both are assets to explore medicinal plants as well as local knowledge to overcome various diseases. Ethnomedicine research by the National Institute of Health Research and Development (NIHRD) of Republic of Indonesia in year of 2012, 2015, and 2017 resulted in local etnopharmacology and medicinal plants in Indonesia. One important information is data on the use of medicinal plants for the treatment of liver disease by traditional healers from various ethnic groups in Indonesia. Analysis of the information set shows that the most widely used plant species for the treatment of liver disease by battra are included in Fabaceae family. Therefore, further studies of the literature regarding the use of empirical, compound content, therapeutic activities and pharmacology of plant species are used as support or even correction for their use in the treatment of liver disease. Various properties as antibiotics (against viruses, bacteria, parasites, fungi), anti-inflammation, antioxidants, hepatoprotectors, and immunomodulators support the use of these species for the treatment of liver disease. Further research is needed to provide basic data on its use in traditional medicine, obtain and develop new drug compounds, and reveal broader use, not to mention toxic and anti-nutritional compounds. This information is expected to be useful for those who are involved in the ethnobotany, botany, pharmacognosy, and pharmacology fields. Abstrak Penyakit liver termasuk salah satu penyakit yang menjadi penyebab kematian utama secara global, dengan angka kematian terus mengalami peningkatan. Hepatitis merupakan salah satu penyakit liver, prevalensi di Indonesia pada tahun 2013 mencapai 1,2%. Sebagai negara megabiodiversitas nomor dua di dunia yang dihuni oleh 1.068 etnis/suku bangsa, Indonesia kaya akan tumbuhan yang dapat dimanfaatkan untuk mengatasi berbagai penyakit. Riset etnomedisin oleh Badan Litbang Kesehatan RI pada tahun 2012, 2015, dan 2017 menghasilkan metadata pengetahuan lokal etnofarmakologi dan tumbuhan obat Indonesia. Salah satu informasi pentingnya yaitu data pemanfaatan tumbuhan obat untuk pengobatan penyakit liver oleh pengobat tradisional (battra) dari berbagai etnis di Indonesia. Analisis terhadap set informasi tersebut menunjukkan bahwa spesies tumbuhan paling banyak digunakan untuk pengobatan penyakit liver termasuk dalam famili Fabaceae. Oleh karena itu, dilakukan studi literatur mengenai pemanfaatan empiris, kandungan senyawa, aktivitas terapeutik dan farmakologi spesies-spesies tumbuhan tersebut sebagai dukungan atau bahkan koreksi terhadap pemanfaatannya untuk pengobatan penyakit liver. Berbagai khasiat sebagai antibiotik (terhadap virus, bakteri, parasit, jamur), anti-inflamasi, antioksidan, hepatoprotektor, dan imunomodulator menyokong pemanfaatan spesies tersebut untuk pengobatan penyakit liver. Penelitian lebih lanjut sangat diperlukan untuk memberikan data dasar penggunaannya dalam pengobatan tradisional, mendapat dan mengembangkan senyawa obat baru, serta mengungkap pemanfaatan yang lebih luas tak terkecuali pula terhadap senyawa toksik dan anti-nutrisi. Informasi ini diharapkan dapat bermanfaat bagi yang menggeluti bidang etnobotani, botani, farmakognosi, dan farmakologi.

Kromatografi cair kinerja tinggi (KCKT) merupakan metode analisis yang dapat dipergunakan untuk menetapkan kadar epigalokatekin galat yang terkandung dalam ekstrak teh hijau. Metode tersebut perlu divalidasi untuk membuktikan bahwa metode tersebut akan memberikan hasil pengukuran yang sesuai dengan peruntukannya. Tujuan penelitian ini adalah membuktikan bahwa metode KCKT yang digunakan akan memberikan selektifitas, linieritas, ketelitian, ketepatan yang memenuhi persyaratan dan diketahui batas deteksi (LOD) dan batas kuantifikasi (LOQ). Pada penelitian ini, selektifitas diketahui dengan menghitung resolusi antar dua puncak yang berturutan. Penetapan kadar 10 ug/ml dan 100 ug/ml dengan replikasi 5 kali dilakukan untuk mengetahui ketelitian (berdasarkan nilai CV) dan ketepatan (berdasarkan nilai perolehan kembali). Linieritas diketahui dengan menghitung nilai r pada kurva hubungan antara kadar dan luas area kromatogram. Dari persamaan kurva baku tersebut juga dapat dihitung nilai LOD dan LOQ. Hasil penelitian menunjukkan bahwa metode yang digunakan memenuhi persyaratan selektifitas dengan nilai Rs 2,27>1,5; cukup linier dengan nilai r= 0,997; memiliki ketelitian dengan nilai CV 8,74% pada kadar 200 µg/ml dan 3,74% pada kadar 500 µg/ml; ketepatan dengan nilai perolehan kembali 99,76% pada kadar 200 µg/ml dan 100,52% pada kadar 500 µg/ml dan nilai LOD sebesar 33,28 ug/ml dan LOQ sebesar 110,93 ug/ml.

Abstrak KLT