In this study determination of a compound commonly known as acetaminophen in the leaf extract of Aquilaria malaccencis was studied and discussed. Aquilaria species are well known for its valuable agarwood. Acetaminophen or 4'-hydroxyacetanilide in its synthesis name is the main compound found in synthetic drugs. This synthetic drug is referred in treating fever, migraine and other type of illness. Traditionally, it is known that Aquilaria species able to treat various illnesses, including fever in which the leaves is treated as tea and believed to cure many other illnesses. A simple and validated method was developed for the study of 4'-hydroxyacetanilide by High Performance Liquid (HPLC). This analysis is well conducted with C18 column to separate the compounds. The method is validated with different parameters in terms of linearity, range, accuracy and robustness. The mobile phase used in this study is acetonitrile and water (20:80). The pH was adjusted to 3.5 with the aid of orthophosphoric acid. The flow rate was set to 0.8 ml/min and a spectrophotometric condition at 193 NM. The percentage of recovery from this method is within 98% to 101%, which indicates this method is reliable and can be applied to an analysis of 4'-hydroxyacetanilide from Aquilaria malaccencis. The concentration of 4'-hydroxyacetanilide determined in the leaves extract of Aquilaria malaccencis at 60 o C were 0.807μg/ml, 1.398μg/ml and 1.811μg/ml for varied ratio ( raw materials: water) 1:80, 1:40 and 1:27 respectively.

Plants are well known for its various uses since ancient times. Plants contribution in various fields such as medical, pharmaceutical and food industry bring a lot of benefits to human capital. Especially in the medical field, plants are major contributors for medicinal drugs as it itself is rich in various medicinal agents. One of the illness that can be treated by using plants is fever. Fever can be treated by consuming non-steroids anti-inflammatory drugs (NSAIDS). However, excessive intake of synthetic drugs bring a lot of harm to the human body especially to the internal organs. Traditionally, various plant is used to cure fever, which directly indicate plants are the best sources of natural antipyretic. Thus, in this present review, activity of natural antipyretic extracted from various plants towards fever will be explained. In this review paper, Google Scholar, PubMed, ScienceDirect were the sources for the published article to collect information regarding antipyretic activity. A number of 32 articles on 10 plant species are found to address antipyretic activities. This review concludes that antipyretic activity can be found indicatively in various plants throughout the world.