Active rehabilitation involves patient's voluntary thoughts as the control signals of restore device to assist stroke rehabilitation. Although restoration of hand opening stands importantly in patient's daily life, it is difficult to distinguish the voluntary finger extension from thumb adduction and finger flexion using stroke patients' electroencephalography (EMG) on single muscle activity. We propose to implement corticomuscular coherence analysis on electroencephalography (EEG) and EMG signals on Extensor Digitorum to extract their intention involved in hand opening. EEG and EMG signals of 8 subjects are simultaneously collected when executing 4 hand movement tasks (finger extension, thumb adduction, finger flexion, and rest). We explore the spatial and temporal distribution of the coherence and observe statistically significant corticomuscular coherence appearing at left motor cortical area and different patterns within beta frequency range for 4 movement tasks. Linear discriminate analysis is applied on the coherence pattern to distinguish finger extension from thumb adduction, finger flexion, and rest. The classification results are greater than those by EEG only. The results indicate the possibility to detect voluntary hand opening based on coherence analysis between single muscle EMG signal and single EEG channel located in motor cortical area, which potentially helps active hand rehabilitation for stroke patients.
Fermented soybean meal (FSM) is an important feed material that can replace fish meal to solve the shortage of animal protein. To improve the utilization of FSM, we optimized the co-fermentation conditions of soybean meal using Bacillus subtilis and Enterococcus faecium and studied the effects of replacing fish meal with different proportions of FSM on serum antioxidant indices and gut microbiota (GM) composition of crucian carp (Carassius auratus). Our results showed that the co-fermentation of soybean meal was the most effective when the ratio of B. subtilis X-2 and E. faecium X-4 was 2:3, glucose addition was 4.5%, KH2PO4 addition was 0.15%, MgSO4·7H2O addition was 0.1%, anhydrous sodium acetate addition was 0.4%, fermentation time was 120 h, and the solid–water ratio was 1:1. Replacing 40% fish meal with FSM in the feed significantly improved the serum T-AOC, POD, and IgM levels in C. auratus. Although there were significant differences in the midgut and hindgut microbiota structures of C. auratus, the addition of FSM to the feed did not cause significant differences in the GM structure, whether in the midgut or hindgut. Therefore, 40% FSM is the most suitable substitute for fish meal in the feed of C. auratus.
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