INTISARIPenelitian ini bertujuan untuk mengetahui kandungan serat kasar dan protein kasar pada bekatul padi yang difermentasi dengan bakteri selulolitik dan jamur selulolitik. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap pola searah dengan sepuluh perlakuan dan masing-masing perlakuan dilakukan 3 kali ulangan. Ke sepuluh perlakuan tersebut adalah P0: 250 g bekatul + 3% tetes + Bakteri (B) 0% + Jamur (J) 0%; P1: 250 g bekatul + 3% tetes +B.10% + J.10%; P2: 250 g bekatul + 3% tetes + B.20% + J.10%; P3: 250 g bekatul + 3% tetes + B.30% + J.10%; P4: 250 g bekatul + 3% tetes + B.10% + J.20%; P5: 250 g bekatul + 3% tetes + B.20% + J.20%; P6: 250 g bekatul + 3% tetes + B.30% + J.20%; P7: 250 g bekatul + 3% tetes + B.10% + J.30%; P8: 250 g bekatul + 3% tetes + B.20% + J.30%; P9: 250 g bekatul + 3% tetes + B.30% + J.30%. Setelah dilakukan fermentasi selama 7 hari, dilakukan analisis proksimat. Data yang diperoleh selanjutnya diuji dengan analisis variansi, dilanjutkan dengan uji Duncan's Multiple Range Test. Hasil penelitian menunjukkan bahwa bakteri selulolitik dan jamur selulolitik dapat menurunkan kandungan serat kasar pada bekatul pada perlakuan P2 (28,96%), P3 (29,34%), P8 (29,53%), P4 (29,65%), P7 (30,23%), P6 (30,37%), P9 (30,58%) yang berbeda nyata (P<0,05) dengan perlakuan P5 (31,10%), P1 (31,98%), P0 (34,11%), serta dapat meningkatkan kandungan protein kasar pada perlakuan P2 (13,97%), P5 (12,87%), P3 (12,84%), P8 (12,74%) yang berbeda (P<0,05) dengan perlakuan P7 (12,71%), P6 (12,44%), P4 (12,36%), P9 (12,27%), P1 (12,25%), P0 (10,9%
Water pollution lately is often becomes an important topic due to its correlation with health. Heavy metals that accumulated in human body mostly come from food. One kind of food that used to be contaminated by heavy metals is fish. Therefore it is interesting to study the heavy metals content in fish, whether it comes from industrial contaminated water. Study on the content of heavy metals Cr, Cu, Pb and Zn in ponds near and far from industrial areas has been conducted. The chosen areas were one from Sidoarjo area and one from Babat area, both are located in East Java. Samples were the meat of fish called tilapia (from Sidoarjo area), and milkfish and goldfish (from Babat area), also the water and mud from the ponds. Analytical method was wet destruction. Measurement of heavy metals content was done by Inductively Coupled Plasma Spectrometer (ICPS). In Babat area’s pond water was not found neither Cr, Cu, Pb nor Zn, while Zn was detected in Sidoarjo area’s, however, it was under the limit of detection. The mud of both ponds contained Cr, Cu, Pb and Zn. The goldfish contained only Cu and Zn, whereas the tilapia and milkfish contained Cu, Pb and Zn, however the metals content were not exceeded the maximum limit stated in the PPOM regulation No. 03725/B/SK/VII/89.
Penyebab kematian lou han Cichlasoma synspilum adalah Aeromonas hydrophila dan Escherichia coli, dengan ciri adanya pendarahan dan luka pada sirip dada. E. coli kemungkinan besar tidak menyebabkan kematian ikan sebab sampai sekarang belum pernah ada laporan bahwa bakteri tersebut menyebabkan sakit pada ikan di mana pun. Gejala klinis utama yang terlihat dari luar adalah pendarahan di sirip dada dan menimbulkan luka yang cukup dalam. Cairan ascites menyebabkan membengkaknya perut, pembengkakan liver, dan kosongnya usus dari ikan yang sakit. Gambaran histopatologi menunjukkan nekrosis dan adanya vacuole pada hepatocyte. Sedangkan pada usus adalah rusaknya mucosal ephitelium, lamina propia stratum compactum, stratum granulosum dan muscularis. Ginjal mengalami kerusakan berat, hampir semua organela ginjal berantakan. Ikan ini dipelihara di aquarium sehingga menurunnya kualitas air atau kontaminasi lewat pakan dan penanganan yang buruk diduga sebagai portal of entry dari penyakit.
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