The drugs moiety included in Biopharmaceutical Classification System Class II and Class IV has low aqueous solubility and remedy for the solubility problem is nanotechnology techniques. Nanotechnology techniques play an important role at the molecular level and nano length scale size. To increase the dissolution rate of drug moiety which directly lead to increase bioavailability of drug depends on decrease drug particles into nano-scale range. Nanotechnology techniques in the pharmaceutical field include nanosuspension. Nanosuspension is biphasic dosage forms contain colloidal dispersions of nanosized drug particles which are stabilized by surface-active agents. Nanosuspension dosage form increases the stability and bioavailability of low aqueous soluble drugs. Solubility plays a crucial role in the effectiveness of drugs irrespective of the route of administration. Most of the presently investigated drug moieties having lower aqueous solubility and therefore has low bioavailability of drugs. The study is focused on formulation consideration, various methods of preparation along with evaluation parameters of nanosuspension.

A combined dose tablet formulation containing Amlodipine besylate and Lisinopril is used for the treatment of essential hypertension. The present study reports development and validation of stability indicating high performance thin layer chromatographic method for simultaneous estimation of these drugs in combined dose tablet formulation. The two drugs were satisfactorily resolved on aluminum plates precoated with silica gel 60F254 using n-butanol : methanol: ammonia (4:4:1 v/v/v) as mobile phase. The Rf value for lisinopril and amlodipine besylate were 0.27±0.02 and 0.62±0.02, respectively. Densitometric evaluation of the separated bands was performed at 215nm. The calibration curves for lisinopril and amlodipine besylate were found to be linear in the concentration range of 1000-6000ng/band. The method was validated as per ICH guidelines for accuracy, precision, robustness, specificity, limit of detection and limit of quantitation. Statistical analysis proves that the method is suitable for simultaneous analysis of Lisinopril and Amlodipine besylate in pharmaceutical formulation without any interference from the excipients/degradant. The developed method offers several advantages such as sensitive, rapid, cost effective and less time consuming as compared to the reported methods. As the method could effectively separate the drugs from its degradation products, it can be employed as a stability indicating method.

The iron-21 syrup is used for iron deficiency anaemia which supplies iron and calories a provide iron and calorie nutriment to recompense haemoglobin deprivation. The objective of this study is to determine acute oral toxicity of Iron-21 syrup in vivo in Wistar rats. Iron-21 Syrup formulation was given through the oral route. The syrup formulation was administered in three increasing doses of 3, 6 and 12 ml/kg body weight for concentration of 500, 1000 and 2000mg/kg respectively. The other group of rats designated as a control group was given the only vehicle orally. The test and control group contains five rats each. Tests, as well as control group rats, were sacrificed on the fifteenth day of treatment. The blood and tissue samples of test animals were sent for histopathological studies examination. Four parameters were observed throughout the study, and they are cage side observation, the effect to the body weight, haematological parameter and histopathology. All animals were survived till they sacrificed. No notable changes were found in behaviour, haematological and histopathology studies. The oral administration of Iron-21 Syrup is not shown any toxic effect in the animal at a given dose. Therefore, it is a safe remedy for human use.

A fatal disorder named oral submucous fibrosis (OSMF) is marked by the oral submucosa's progressive fibrosis. OSMF is distinguished by aberrant collagen deposition. In 1.5-15% of patients, it's a precancerous condition that progresses to a malignant tumour. Although nutrient deficiencies and immunological procedures could perform a role in pathogenesis, epidemiological evidence suggests that betel nut quid (which contains guvacine, arecoline, guvacoline, arecaidine, and chavibetol) contains areca nut, slaked lime, tobacco, is a significant risk factor for OSMF. Submucous fibrosis, xerostomia, ulceration, a burning sensation, and a limited mobility of the mouth are among the symptoms. The patient's quality of life is seriously affected by each of these components. The present article gives a broad overview of OSMF from a molecular viewpoint and describes what has been learned about its underlying causes, methods of diagnosis, and available treatments. Along with active therapy for OSMF, prophylaxis is essential, and this section gives a quick review of its treatment.