Chronic suppurative otitis media (CSOM) is an important cause of preventable hearing loss, particularly in the developing world. Aim: To determine the microbiological profile of CSOM and determine the antibiotic susceptibility testing of aerobic bacteria which will be beneficial for appropr iate treatment there by reducing complications. Methods and Materials: A total of 109 patients with CSOM attending outpatient and admitted inpatient in ENT department were included in the study. Two pus swabs each for infected ear were collected. Specimens were subjected to Gram stain and plated on to appropriate culture media to isolate bacterial and fungal pathogens. Bacterial and Fungal species identification and Antibiotic susceptibility testing of aerobic bacteria of isolated pathogens was done. Results: Out of 109 samples cultured there were 70 bacterial and 14 fungal isolates. Most common aerobic bacterial isolate was Pseudomonas aeruginosa (34.2%) followed by Proteus mirabilis (22.8%) and Staphylococcus aureus (17%). Among fungal isolate, Aspergillus niger (64.3%) was predominant followed by Aspergillus flavus (21.4%) and Candida species (14.3%). Amikacin (91.6%) and Ciprofloxacin(87.5%) showed maximum activity to Pseudomonas aeruginosa. The antibacterial susceptibility pattern of Staphylococcus aureus revealed 58.3% Methcillin resistant Staphylococcus aureus (MRSA) strains. Conclusions: Emergence of Pseudomonas aeruginosa as most common aerobic isolate and high incidence of Methicillin Resistant Staphylococcus aureus in present study indicates the necessity of continuous evaluation of bacteriological profile and antimicrobial susceptibility testing in all CSOM cases.