Purpose of the study. To study the balance of fatty acid spectrum of oral lipids as a marker of oxidant-antioxidant stress in patients with generalized parodontitis with anorexia nervosa. Materials and methods. The study metabolic disorders of fatty acids oral fluid was conducted in 28 patients with GP, primary-I, I–II degree, chronic course associated with anorexia nervosa, (average age 26 ± 3,8) – the main group (M). The comparison group (C) included 23 patients with GP, primary-I, I–II degree, chronic course with no eating disorders. The control group consisted of 25 healthy individuals of the same age category. Patients' oral fluid was used as the object of study. In the spectrum of fatty acids of lipids of the oral fluid, the 9 most informative fatty acids were identified: myristic C 14:0,pentodecane C 15:0, palmitic C 16:0, margarine C 17:0,stearic C 18:0, oleic C 18:1, linoleum C 18:2, linolen C 18:3, arachidone C 20:4. Quantification of the spectrum of lipid fatty acids was performed by the method of normalization of planes and determined the proportion of acids in percents. Results. The fatty acid content of the oral fluid of patients in the main group was significantly different from the control parameters. 12% in patients with anorexia nervosa significantly increased the amount of saturated fatty acids by increasing the content of palmitic acid (C 160) and reducing stearic acid (C 18:0) by 2 times compared with control.It was established that the sum of unsaturated fatty acids was reduced by 2 times due to polyunsaturated fatty acids, and this level was significantly reduced by 2 times in comparison with control group due to 3 times reduction of linoleum fatty acid (C 18:2), linolenic fatty acid (C 18:3) 2-fold and unreliable increase in arachidone fatty acid (C 20:4), in comparison with the control, which in our opinion, reflects disturbance of the oxidant-antioxidant balance and is a marker of stress as an indicator of endogenous intoxication in patients with GP and anorexia nervosa.In patients with generalized parodontitis of primary-I, I–II degree, chronic course of anorexia nervosa in the oral fluid there was a significant violation of lipid metabolism – increase in the content of palmitic fatty acid (C 16:0) and decrease in the content of linoleic fatty acid (C 18:2). Conclusions. The revealed features of the course of GP in patients with anorexia nervosa indicate the need for additional treatment and prophylactic measures in this group of patients. Disorders of the fatty acid spectrum of the oral fluid in these patients were necessitate the inclusion in the traditional scheme of treatment of GP aimed at correcting the processes of antioxidant protection, which increase the effectiveness of therapeutic and prophylactic effects. Keywords: generalized parodontitis, anorexia nervosa, fatty acids, oral fluid, oxidative-oxidant stress.

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Relevance. Alloxan and streptozotocin are used for experimental modeling of hyperglycemia. Damaged β-cells with these compounds lead to the reduction of synthesis and secretion of the level of insulin in the blood, as a result of which animals develop hyperglycemia and diabetic syndrome, similar to insulin-dependent type 1 diabetes. One of the methodological approaches for assessing the degree and development of tissue damage of vital organs, including diabetes, is the study of their fatty acid composition. But for the planning of an experiment with type 1 diabetes, it is necessary to take into account the discrepancy between the data obtained on different models. Objective. The aim of the work is to study the changes in the fatty acid composition of cardiomyocyte and hepatocyte lipids in type 1 diabetes of the rats which are created on different models: alloxan (Allox) and streptozotocin (STZ). Material and methods. In the experiment, the model of type 1 diabetes was developed by the single-dose administration of streptozotocin (50 mg/kg) and alloxane monohydrate in a dose of 130 mg/kg to white rats. Their administration caused a persistent hyperglycemia (20-34 mmol/L) to the majority of experimental animals. Research of the contents of 9 most informative fatty acids (FA) in the composition of the myocardium and liver homogenate was carried out by gas-vapor chromatography in the experimental laboratory of Scientific research institute. The statistical significance of differences in the meanings of the groups of comparisons was estimated by the Student's t-criterion. Results. In the Allox model, we discovered a significant (p<0,05) 2-fold decrease in the proportion of palmitic FA to 9,5±0,5% relative to the control group 18,6±1,0% and stearic liquid crystal to 5,5±0,6% relative to the control 11,1±1,0% in the hepatocytes than in to STZ model. Additionally, we observed, a 12-fold increase in the content of meristic FA and pentadecane FA from 0,1±0,05% in the control to 1,2 ±0,1% in rats with the Allox model of diabetes. Among the unsaturated FA a significant increase (p <0,05) of 2,4 times the growth of oleinic FA from 14,8±1,5% to 34,2±1,6%, and a significant (p<0,05) in 1,5 times reduction of arachidone FA from 30,1±1,5% in control to 19,6±1,0% in rats with diabetes in the liver could be noted. A significant increase (p<0,05) of oleinic FA from 15,1±0,7 in the control to 33,1±1,5% and a decrease of 1,3 times the linoleic FA from 28,8±1,0% in control to 21,8±2,0% in the Allox model of rats with 1 type diabetes was showed in the myocardium. On the other hand, the STZ model rats increased the content of linoleic acid to 34,9±1,0%, which was a significant (p<0,05) difference with the control and Allox model. It is believed that the discrepancy in data is due to different pathogenetic mechanisms of action of drugs on tissues, which become targets of damage. Conclusions. The Allox model of type 1 diabetes shows the aggressive effects of high-level radicals and can be successfully used to study the mechanisms of oxidative stress, which necessarily occurs in conditions of hyperglycemia. The development of the STZ model of diabetes reflects the less awful effects of cell's damage, and such simulations should be used in the study of tissue and organ disorders that occur with prolonged and persistent hyperglycemia.