No abstract

Multiple genotypically unique strains of the tick-borne pathogen Anaplasma marginale occur and are transmitted within regions where the organism is endemic. In this study, we tested the hypothesis that specific A. marginale strains are preferentially transmitted. The study herd of cattle (n ‫؍‬ 261) had an infection prevalence of 29% as determined by competitive inhibition enzyme-linked immunosorbent assay and PCR, with complete concordance between results of the two assays. Genotyping revealed the presence of 11 unique strains within the herd. Although the majority of the individuals (70 of 75) were infected with only a single A. marginale strain, five animals each carried two strains with markedly distinct genotypes, indicating that superinfection does occur with distinct A. marginale strains, as has been reported with A. marginale and A. marginale subsp. centrale strains. Identification of strains in animals born into and infected within the herd during the period from 1998 to 2003 revealed no significant difference from the overall strain prevalence in the herd, results that do not support the occurrence of preferential strain transmission within a population of persistently infected animals and are most consistent with pathogen strain transmission being stochastic.Anaplasma marginale is the most globally prevalent ticktransmitted pathogen of livestock, with regions of endemicity on all six permanently populated continents (13). In North America, A. marginale exhibits dramatic strain diversity, with numerous distinct genotypes having been identified in the past 15 years (1,5,6,8,14). As A. marginale is not passed transovarially in the tick and thus cannot be maintained between generations, transmission requires the presence of infected mammalian reservoir hosts (12,17). Consequently, the presence of multiple distinct strains within the population of infected reservoir hosts provides potential diversity for transmission. A previous study in a region of eastern Oregon where the organism is endemic identified six closely genetically related A. marginale strains within a herd of persistently infected cattle, an observation consistent with ongoing transmission of multiple strains within the herd (14). Independent transmission of multiple strains within a herd is supported by the isolation of distinct A. marginale strains, each from an individual cow with acute anaplasmosis, within a period of several months (7). It is unknown whether transmission of a specific A. marginale strain is stochastic, merely reflecting the strain composition present in the herd, or whether there is preferential transmission of specific strains. The identification of both qualitative (transmissible or not by a specific tick vector) and quantitative (strain-specific differences in the number of organisms per infected tick) differences in transmissibility among A. marginale strains provides a basis for preferential transmission (2,9,16,19).To test the hypothesis that specific A. marginale strains are preferentially transmitted within a her...

Milk EPD, used to predict the milk production potential of a parent's daughters, have been reported by all major cattle breed associations. Our objectives were to determine the relationship of milk EPD of a dam to actual milk production (both fluid and components) and offspring weaning weight. Angus (AN; n = 114) and Simmental (SM; n = 82) cows were machine-milked at approximately 60, 104, and 196 d postpartum after overnight calf removal. In addition, one herd of AN was also milked at approximately 35 and 145 d postpartum. A lactation curve was fitted to these measurements to estimate total milk production during lactation. Simple correlations between 205-d total milk yields (TMY) and adjusted 205-d calf weaning weight (WW) were .30 (P < .001) and .47 (P < .001) for AN and SM, respectively. Furthermore, milk EPD was positively correlated to adjusted WW (r = .38 P < .001; r = .39, P < .001) and TMY (r = .32, P < .001; r = .44, P < .001) for AN and SM cows, respectively. A 1-kg change in TMY changed WW by .014 +/- .006 kg (P < .001) in AN and by .032 +/- .009 kg (P < .001) in SM. A 1-kg change in milk EPD resulted in a 4.85 +/- 1.14 kg change in WW (P < .001) in AN and a 3.74 +/- 1.73 kg (P < .05) change in SM. Corresponding changes in TMY were 42.1 +/- 16.6 kg (P < .01) and 69.3 +/- 16.0 kg (P < .001) for AN and SM, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

In three consecutive years, spring-calving Hereford and Hereford x Angus cows (n = 348) were used to determine effects of level of supplemental energy or protein before and after calving on cowherd performance. Beginning on November 1, cows were individually fed 1.22 kg/d of a 40% CP (PROTEIN) or 2.44 kg/d of a 20% CP supplement (ENERGY) until calving. After calving, cows remained on the same supplement, were switched to the other supplement, or were fed 2.44 kg/d of a 40% CP supplement (HI PROT). Supplementation ended on April 20, the start of a 65-d breeding season. Cows fed ENERGY during gestation had greater BW gains (9 kg) at calving than PROTEIN-fed cows (P < .01). Calf weaning weight was not affected by supplementation. Cows fed ENERGY before calving had an 11% greater pregnancy rate than the cows fed PROTEIN (P < .002). Cows fed PROTEIN or ENERGY after calving had similar BW gains but cows fed HI PROT after calving lost less BW during supplementation (P < .002). Pregnancy rates were not influenced by treatments fed for a short period after calving. In conclusion, conception rates were significantly improved by feeding greater levels of supplemental energy prepartum but not postpartum. Energy supplements can affect reproduction with minimal effects on BW or condition.

Sixty crossbred cull cows were used to determine the combined effects of a trenbolone acetate-estradiol implant and feeding zilpaterol hydrochloride on performance, carcass characteristics, and subprimal yields of mature cows fed for 70 d. Cows were assigned to 1 of 5 treatments: 1) grazing native grass pasture (G); 2) concentrate-fed (C) a grain sorghum-sorghum silage diet; 3) concentrate-fed and implanted (CI) with Revalor-200 (trenbolone acetate-estradiol); 4) concentrate-fed and fed Zilmax (zilpaterol hydrochloride) beginning on d 38 of the feeding period (CZ); and 5) concentrate-fed, implanted, and fed Zilmax beginning on d 38 (CIZ). The concentrate diet consisted primarily of ground grain sorghum and sorghum silage. During the last 34 d of the feeding trial, concentrate-fed (C, CI, CZ, and CIZ) cows had greater (P < 0.05) gains than G cows. Hot carcass weights and dressing percentages were greater (P < 0.05) for the concentrate-fed cows than for G cows. Longissimus muscle area was largest (P < 0.05) for CIZ cows, whereas subprimal weights from the chuck were heavier (P < 0.05) from CIZ cows than C and G cows, and carcasses from CI and CZ cows had heavier (P < 0.05) chuck subprimal weights than G cows. Rib and round subprimal weights were heavier (P < 0.05) for concentrate-fed cows compared with G cows. In addition, carcasses from CIZ cows had heavier (P < 0.05) total subprimal weights, and total subprimals were a greater percentage of their initial BW than C cows. Rib cut-out and total soft tissue weights from the 9-10-11th rib were less (P < 0.05) for G cows than concentrate-fed cows. Feeding cull cows a concentrate diet increased carcass weight, dressing percentage, and subprimal yields compared with feeding cows a grass-based pasture diet, and the combination of a trenbolone acetate-estradiol implant and feeding zilpaterol hydrochloride can maximize trimmed beef yields from cull cows fed a high-concentrate diet.