Patients with PCOS as diagnosed based on the Japanese diagnostic criteria include those with increased and normal androgen levels. LOD is considered an effective ovulation induction method for both types of patients with PCOS.

Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol–aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating.

Magnetostriction of an Fe94Si6 (at. %) single-crystal is investigated under rotating magnetic fields and the behavior is analyzed by using a modified model of coherent rotation of the magnetization. The Fe-Si(001) single-crystal film has the magnetic easy axes lying along <100> and shows a four-fold anisotropy with the magnetic anisotropy field and the coercivity of 450 and 5 Oe, respectively. The magnetization structure changes from multi-magnetic domains to a single-magnetic domain with increasing the magnetic field beyond 10 Oe. The shape of magnetostriction output measured along bcc [110] as a function of magnetic field rotation is triangular and the amplitude increases with increasing the magnetic field up to 0.5 kOe and then saturates. The behavior is analyzed by employing the modified coherent rotation model where the magnetization reversal field employed in the analysis is determined experimentally. The calculated results show good agreements with the experiments, which indicates that the magnetostrictive behavior can be analyzed by taking into account the magnetocrystalline anisotropy which is easily estimated from the magnetization curve. The calculated magnetostrictive behavior along [100] shows a bathtub-like waveform and the amplitude stays at almost constant even when the magnetic field is increased up to the anisotropy field, where the magnetostrictive behavior is quite different from that measured along [110].

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