The current experiment was aimed to explore the influencing of the sex-linked dwarf gene on some fertility hormones and biochemical profile in normal local and dwarf roosters. Thirty normal local and dwarf roosters at age 28 weeks were housed at opened system for 6 weeks. At end of 6th week, blood samples collected from all roosters and centrifuged to obtained sera for measuring the concentrations of Interstitial cell stimulating hormone ‘ICSH’, Follicle stimulating hormone ‘FSH’ and testosterone hormone, total protein, albumen, globulin, glutamic oxaloacetic transaminase ‘GOT’, Alkaline phosphatase ‘ALP’, and glutamic pyruvic transaminase ‘GPT’. The statistical analysis documented significant (P<0.05) decline in FSH and testosterone hormone in dwarf group roosters compared with normal local roosters. As well as, the level of total protein and globulin registered significant decline (P<0.05) in dwarf group roosters in comparison with normal local group roosters. On the other hand, the level of hepatic enzymes, GOT, GPT and ALK, recorded a significant (P<0.05) elevation in dwarf group roosters when compared with normal local group roosters. Concluded, that the sex-linked dwarf gene could be affected on hormonal fecundity profile and some biochemical traits in dwarf roosters.