He et al. show that T reg deficiency markedly induces autoimmunity and shifts gut microbiota. Remodeling microbiota by Lactobacillus reuteri was found to inhibit autoimmunity via the metabolite inosine, which interacts with the adenosine A2A receptor. This finding establishes a link between the gut microbiota, A2A receptors, and autoimmunity induced by T reg cell deficiency.

The relative persistence of behavior can be efficiently measured by dividing the response rate under the PR schedule by the response rate under the FR schedule. Measures of relative persistence corresponded well with measures of choice and show that relative reinforcing effects increase as dose increases.

This report describes an anesthesia technique that we used to study cardiovascular anatomy and physiology with echocardiography and cardiac magnetic resonance (CMR) in 46 African clawed frogs (Xenopus laevis) (n = 24 for electrocardiography and n = 22 for CMR). For administration of anesthesia, 3 holding tanks, one each for transportation, sedation, and recovery, were filled with filtered water, with 0.05% buffered tricaine methasulfonate solution (MS-222) added into the sedation tank. Fifteen minutes after the frog was placed in the sedation tank, a paper towel was soaked in MS-222 solution, and the frog was placed in a supine position and rolled 3 to 4 times in the soaked paper with the head and legs exposed. Vital signs were monitored and recorded throughout the procedure. After imagining, frogs were unrolled from the paper towel, placed in the recovery tank, and later returned to their home tank. Monitoring was discontinued when the frogs resumed typical activity. No mortality or complications were observed in frogs that underwent this procedure. Mean duration ± 1 SD of anesthesia induction was 12 ± 5 min in the echocardiography group and 14 ± 6 min in the CMR group. The mean duration of anesthesia maintenance was 60 ± 18 min in the echocardiography group and 118 ± 37 min in the CMR group. An additional dose of anesthesia was necessary during maintenance for 9 of 24 (37%) frogs in the echocardiography group and 6 of 22 (27%) frogs in the CMR group. At the end of the procedure, the mean oxygen saturation was 66 ± 9% in the echocardiography group and 85 ± 6% in the CMR group, and heart rate was 48 ± 13 beats/min in the echocardiography group and 42 ± 7 beats/min in the CMR group. We conclude that the anesthesia technique of immersion in MS-222 is suitable for performing echocardiography and CMR imaging in this species without complications.

Choice behavior was studied under concurrent nonindependent fixed‐ratio fixed‐ratio (nFR) schedules of reinforcement, as these schedules result in frequent changeover responses. With these schedules, responses on either operandum count toward the completion of the ratio requirements of both schedules. Five monkeys were subjects, and two pairs of liquid reinforcers were concurrently available: 16% (w/v) and 0% ethanol or 16% and 8% ethanol. For each pair of reinforcers, the nFR sizes were systematically altered across sessions while keeping the schedule size equal for both liquids. Responding varied as a function of reinforcer pair and nFR size. With the 16% and 0% pair, higher response rates were maintained by 16% and were an inverted U‐shape function of nFR size. With 16% and 8%, a greater number of responses initially occurred on the schedule that delivered 8% ethanol. However, as nFR size increased, preference reversed such that responses that delivered 16% ethanol were greater. When the nFR size was subsequently decreased, preference reverted back to 8%. Number of responses emitted per delivery was a dependent variable and, in behavioral economic terms, was the price paid for each liquid delivery. With 16% and 0%, changeover responses initially increased and then decreased as schedule size became larger. In contrast, with the 16% and 8% pair, changeover responses increased directly with schedule size. Responding under nFR schedules is sensitive to differences in reinforcer magnitude and demonstrates that relative reinforcing effects can change as a function of schedule size.

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