A slmple effective flow fluorometer has been assembled and operated In conjunctlon wlth a flow Injection analysls (FIA) system. A submlcrollter caplllary sheath flow cell Is used for fluorescence detection wlth a laser source. The Instrument has been tested for stablllty, preclslon, accuracy, sensitivity, dynamic range, and speed. Rhodamlne 6G was used as a standard fluorophore. Appllcatlon was also made to the cllnlcal analysis of oxidase enzyme dependent reactlons by reactlng H202 with leucodlacetyldlchlorofluoresceln to produce fluorescent dichlorofluoresceln. Thls produced dye is excited at 488 nm wlth the argon laser. Concentratlons of lo-'' M hydrogen peroxide could be detected when Injecting 25 pL allquots.
Using anA new triglyceride or glycerol measurement system is described based on the indirect electrochemical monitoring of NADH via its reaction with oxygen by horseradish peroxidase. Derivative amperometric signals due to oxygen depletion provide for a one-point kinetic analysis. Lipase hydrolyses triglycerides to glycerol and glycerol dehydrogenase catalyses the reaction of glycerol with NAD to produce NADH. Serum measurements are demonstrated.
In 2003, the Association of American Medical Colleges (AAMC) stopped using the term "underrepresented minority" and instead adopted "underrepresented in medicine." This was not the first time the AAMC revised this definition. In this article, the authors call on the AAMC to revise and expand this definition to include another group that is underrepresented in medicine: lesbian, gay, bisexual, transgender, and queer (LGBTQ) individuals. It is difficult to know whether LGBTQ populations are underrepresented in medicine; however, the data that do exist suggest a significant lack of LGBTQ representation in medicine. It is unclear if this underrepresentation is due to a true numerical lack of LGBTQ physicians, to LGBTQ physicians not publicly selfidentifying due to anti-LGBTQ sentiments and reactions, or to both.
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