Amperometric determination of glycerol and triglycerides using an oxygen electrode

Abstract

Using anA new triglyceride or glycerol measurement system is described based on the indirect electrochemical monitoring of NADH via its reaction with oxygen by horseradish peroxidase. Derivative amperometric signals due to oxygen depletion provide for a one-point kinetic analysis. Lipase hydrolyses triglycerides to glycerol and glycerol dehydrogenase catalyses the reaction of glycerol with NAD to produce NADH. Serum measurements are demonstrated.

Determination of serum triglyceride by enzyme electrode using covalently immobilized enzyme on egg shell membrane

“…Immobilized enzyme based biosensors are comparatively more simple, sensitive and rapid. There are few enzyme based TG biosensors, such as amperometric biosensor based on collagen membrane [7] amperometric biosensor using dissolved oxygen electrode [8,9], potentiometric biosensor using porous silicon [10], amperometric biosensor using prussian blue modified screen printed electrode [11]. Other amperometric triglyceride biosensors are based on artificial membrane bound enzymes such as collagen, [7] CA [14] and PVC [12] membranes.…”

Analysis of free glycerol in biodiesel using an electrochemical assay based on a two-enzyme platinum microelectrode system

“…1. Several amperometric sensors have been described in the literature that take advantage of this oxygen consumption [7,9,10] and hydrogen peroxide production presented in reactions 1 and 2 [3][4][5].…”

Analysis of Free and Total Glycerol in Biodiesel Using an Electrochemical Assay Based on a Two‐Enzyme Oxygen‐Electrode System

“…Several amperometric sensors have been described in the literature based on the oxygen consumption and hydrogen peroxide production as presented in Reactions 1 and 2 of Figure 1 [1,12,14,15]. All of these glycerol analysis studies have been performed in matrices such as tobacco casing, fermented products, blood serum and plasma.…”