<p>A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 <sup>0</sup>C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. <em>Bacillus </em>sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24<sup>th</sup>, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (<em>Hermetia illucens</em>) have competencein organic waste degradation, because in Black Soldier Flays<em> Larvae’s</em> gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially <em>Bacillus </em>sp,</p><p><strong>How to Cite</strong></p><p>Supriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (<em>Hermetia illucens</em>) Feeding with Rice Straw. <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(3), 314-320. </p>
Basically, education is a structured and systematic pattern that aims to improve human civilization, including biology teaching which is expected to contribute to the improvement of student behavior. Therefore, it is necessary to integrate religious values as an important part in biology lessons. This research aims to know the effect of applying faith and taqwa (IMTAQ) approach to Biology learning in improving meaningful learning, with learning outcomes in the form of conceptual mastery and affective improvement. The research was conducted by using quasi experimental method, which is expected to describe phenomenon, occurrence, and causal relationship as the impact of integritation value of religious on biology subjects in the school of MAN 2, Bandung. In the research focus, it is assumed that teachers have the ability to integrate general science materials with Islamic values. The results showed that the integration of Islamic values through IMTAQ approach can improve the process and the students learning outcomes, significantly. Used of this approach can also improve teacher performance with three reasons; 1) learning time more effective and efficient, 2) learning more focused on analysis of study of general and religious knowledge, and 3) learning activities more controlled. Through this model, the acquisition of learning outcomes has increased the mastery of significant student concepts, as evidenced by the increasing grade of students of class XI IPA from pre-test (36) and post-tests (74) results, with N-Gain 0.58 (medium category). The average post-test score indicates that 80% have reached of KKM (70), whereas affective indicator is 4.24% (high qualification). This study demonstrates that the integration of Islamic values through an approach of faith and taqwa in biology learning can improve the meaningfulness of learning, and similar concepts can be implemented in other subjects and other schools.
Hasil belajar siswa pada mata pelajaran biologi di SMAN 24 Kota Bandung senantiasa dilakuakn upaya peningkatan kualitas hasil pembelajaran.Hal tersebut dilihat dari nilai ulangan harian, seperti pada konsep system reproduksi pada wanita.Tujuan penelitian ini adalah untuk mengetahui hasil belajar siswa baik kognitif, afektif dan psikomotor siswa serta peningkatan hasil belajar mereka setelah pembelajaran berlangsung dengan menggunakan pendekatan ekspositorisub materi pokok sistem reproduksi wanita. Metode yang digunakan adalah Penelitian Tindakan Kelas (PTK) bentuk kolaboratif parsipatoris.Hasil penelitian terhadap hasil belajar siswa dengan menggunakan pendekatan ekspositori pada sub materi pokok sistem reproduksi wanita diperoleh data yaitu (1) Hasil belajar kognitif siswa berdasarkan pretes dengan nilai 31.9, sedangkan hasil belajar postes dengan nilai 59.6. Hal tersebut menunjukkan bahwa terdapat peningkatan hasil belajar secara signifikan karena thitung = -10.392 < tdaftar = 2.656, dengan rata-rata nilai gain sebesar 28. (2) Sikap siswa setelah proses pembelajaran berlangsung menyatakan bahwa sebagian besar siswa (52.12%) setelah proses pembelajaran menunjukkan sikap senang terhadap pendekatan ekspositori,35.88% siswa kurang menyukai terhadap pendekatan ekspositori dan 12.00% siswa tidak menyatakan sikap (ragu) terhadap pendekatanekspositori setelah pembelajaran berlangsung. (3) Keterampilan siswa berkembang ke arah positif, terlihat dari rata-rata keterampilan siswa siklus I sebesar 61.0%, siklus II sebesar 80.0% dan keterampilan siswa pada siklus III sebesar 91.5%, (4) Hasil belajar siswa dari ketiga aspek terlihat adanya peningkatan hasil belajar demikian pula dengan sikap dan keterampilan mereka berkembang kearah positif, hal tersebut didukung dari hasil observasi dan wawancara siswa selama proses pembelajaran.
Chicken meat is one of the primary protein sources for the Indonesian market. However, due to the warm and humid climate, chicken meat is subject to microbial contamination, i.e., Escherichia coli dan Salmonella sp. The source of the contamination is the chicken gastrointestinal environment during rearing and post-harvest handling. One of the approaches to solve this problem is applying the natural product, which is safe for human consumption, as an antibiotic and preservation agent. This study used propolis of Tetragonula laeviceps as a feed supplement and preservation agent for local chicken meat. Chickens were divided into three groups (1) group I, in which chicken did not provide with propolis supplement and the harvested meat did not dip into propolis (2.5%), (2) group II in which chickens provided with propolis supplement and the harvested meat did not dip into propolis (2.5%), (3) group III in which chicken provided with propolis during rearing and the harvested meat did not dip into propolis solution, and (4) group IV in which chicken provided with propolis supplement (propolis 3%) during rearing and harvested meat dipped into propolis. Observation of Escherichia coli dan Salmonella sp. Infestation conducted at 0, 4, and 8 h. The result indicated that 2.5% propolis solution suppressed E. coli for all observation periods. Still, the application of propolis during the chicken-rearing period did not significantly reduce the E. coli population in the meat. Both Triple-Sugar-Ion (TSI) and Lysne Iron Agar (LIA) tests did not detect any Salmonella sp infestation. However, the disc diffusion method showed antibacterial activity of propolis against Salmonella thyprinum. The present finding suggested that Indonesia local chicken is more susceptible to E. coli infestation than Salmonella and the application of propolis, as immersion agent, may slowing the population growth on the meat.
Waste of cajuput leaves (Melaleuca cajuputi Powell) has the potential to be used as an alternative cattle feed because it has a fairly high nutritional content. The nutritional content of cajuput leaf waste needs to be improved before being used as an alternative feed. The fermentation process can increase nutrient content in cajuput leaf waste and increase the digestibility of organic matter (OMD), dry matter digestibility (DMD), Ammonia (NH3), and Volatile Fatty Acid (VFA). This study aimed to determine the improvement of cajuput leaf waste nutrition with the help of yeast as well as the effect of fermented cajuput leaf waste along with a mixture of concentrates and field grass on digestibility, NH3, and VFA which were carried out in vitro test. The results showed the nutritional content of cajuput leaf waste fermented by yeast of Aspergillus chevalieri had the best value of protein 16.03%, fiber 16.92%, and fat 5.93%. The treatment R4 (50% cajuput leaf waste + 25% concentrate + 25% field grass) had the best digestibility (DMD=46.12%; DMO=32.08%), NH3 (8.37mM) and VFA (168.5mM) production.
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