Herbal products are preferable to synthetic medicines, and the use of traditional medicines is increasing day-by-day. The current study was designed to evaluate the potentials of bioactive compounds from Citrullus colocynthis by performing FTIR, HPLC, and GC-MS analyses, which explore the good concentration of the secondary metabolites, such as gallic acid (74.854 ppm), vanillic acid (122.616 ppm), and ferulic acid (101.045 ppm) with considerable bioactivities. Antimicrobial protein was estimated by performing SDS-PAGE, ranging from 15 to 70 kDa in all protein fractions. The current study also checked the cytotoxicity of the bioactive compounds in the active fraction of C. colocynthis, and to perform this activity, the groups of rats were arranged with 16 rats randomly divided into four groups (three experimental and one control) by administering various dosage of methanolic fractions in dose-dependent manner. Histopathology was conducted on the livers of the rats after 15 days of sacrifice under deep anesthesia. In liver cell slides examined at the maximum dose of 600 mg/kg, minimal morphological changes, such as slight ballooning, nuclear variation, vacuolar degeneration, and hydropic degeneration, were observed. Furthermore, the in silico analysis identified bioactive compounds as potential drug candidates.

Salvadora persica, commonly recognized as the Miswak tree, happens in shrub savannah. From northwestern areas. The current evaluation gives a complete summary of the chemical materials and organic results (antibacterial and anti-parasitic specially scabies) of this species.To examine the qualitative and quantitative analyses of methanolic and ethanolic extracts to screen the phytochemical contents, sulfur containing compound present in Salvadora persica against secondary bacterial infection of scabies. Numerous compounds were discovered using GCMS analysis, biological activities, Molecular docking and anti-bacterial activity of leaf extracts. It contributes to widespread disease brought on by secondary infections and post-infectious conditions such acute post-streptococcal glomerulonephritis Sarcoptic scabies mites' variant Hominis can be killed by sulfur-containing compounds. The extract of the particular elements that have an effect against scabies and the subsequent bacterial infection inside the single dose. They can protect against secondary bacterial scabies infections. The potential interaction and binding affinity between ligands2-(2 Methyl vinyl) thiophene, Benzene, (Isothiocyanatomethyl) and Benzyl Nitrile and protein (3h7t) were analyzed by Auto dockvina. The ligand tended to bind with the binding energy − 4.3kcal/mol, -5.4kcal/mol and − 5.7kcal/mol respectively.