The aim of this work was the determination of rat thymocytes response to hypergastrinemia evoked by hypoacidity and multiprobiotic «Symbiter® acidophilic concentrated» (symbiter) treatment via the estimation of the interferon (IFN) titer and 2', 5'-oligoadenylate (OA)-synthetase activity in lymphocytes. 2', 5'-OA-synthetase is the IFN-induced enzyme. Methods. The micromethod of IFN titer determination by antiviral activity, spectrophotometrical method of 2', 5'-OA-synthetase activity determination. Results. It was shown that the IFN production by cultivated thymocytes is amplified while the 2', 5'-OA-synthetase activity decreases in these cells in conditions of hypoacidity caused by the 28-days omeprazol treatment. The treatment of animals by symbiter against a background of hypoacidity causes the augmentation of IFN production by thymocytes, but does not stimulate the 2', 5'-OA-synthetase activity. The IFN production by thymocytes in response to IFN inducers (PHA and cycloferone) in vitro is intensified comparatively to the control at hypoacidity and symbiter treatment. Conclusions. The multiprobiotic symbiter exhibits interferonogenic properties. The IFN synthesis in response to induction in vitro is intensified in comparison with healthy animals at both hypoacidity and symbiter treatment while the 2', 5'-OA-synthetase acivity in thymocytes decreases

Вивчено продукцію інтерферону (ІФН) лімфоцитами тимусу щурів і активність ІФН-індукованого ферменту 2′,5′-олігоаденілат-синтетази (2′,5′-ОА-синтетази) за умов гіпоацидності шлункового соку, викликаної введенням омепразолу, а також за сумісної дії омепразолу та мультипробіотика «Апібакт®». Встановлено, що на фоні тривалої гіпоацидності шлункового соку продукція ІФН тимоцитами посилюється у 2,5±1,2 разу порівняно з контролем (інтактними тваринами), при цьому активність 2,5′-ОА-синтетази знижується на 28±1,34%. У тимоцитах тварин із гіпоацидністю, стимульованих фітогемаглютиніном А (ФГА) (20 мкг/мл) і циклофероном (50 мкг/мл) in vitro, збільшується продукція ІФН й активність даного ферменту порівняно з не-стимульованими клітинами цих же тварин. «Апібакт®» на тлі введення омепразолу посилює дію ФГА на продукцію ІФН та 2′,5′-ОА-синтетазну активність у тимоцитах. Ймовірно, за умов гіпоацидності шлункового соку стимулюється синтез ІФН лімфо-цитами тимусу, що може відбуватися внаслідок активації імунної відповіді за коло-нізацію шлунка умовно-патогенною мікрофлорою. «Апібакт®» проявляє імуномоду-люючі властивості, збільшуючи здатність лімфоцитів тимусу до синтезу ІФН у відпо-відь на екзогенну стимуляцію у щурів з гіпоацидністю шлункового соку.Ключові слова: гіпоацидність, омепразол, мультипробіотики, «Апібакт®», тимоцити, інтерферон, 2′,5′-олігоаденілат-синтетаза, індук-тори інтерферону. ВСТУПТривала гіпоацидність шлункового соку призводить до дисбактеріозу та розвит-ку запальних процесів у різних відділах травного тракту: ротова порожнина

Purpose of the study. The sepsis differentiation criteria and metabolically induced diabetic foot lesions must be established. Material and methods. The 115 patients were observed. Results and discussion. Sepsis by the qSOFA criteria was diagnosed in 3, heart failure of 3–4 class by NYHA classification estimated in 39 cases. High limb amputation had been performed to 18 patients, surgery on the foot to 97. Foot lesion relapse accompanied by unstable glycaemia had been observed in 25 cases. For the surgical correction of the diabetes ileoduodenoplasty had been performed in 7 cases, which resulted in uncomplicated wound healing in all patients. Conclusion. Sepsis progression in patient with diabetic foot case proof indicates the necessity high lower limb amputation. Foot lesion relapse after the effective surgical sanitation indicate on metabolic disorder, not on sepsis. Surgical correction of the diabetes by mean of ileoduodenoplasty performing seems to be the reliable method of foot lesion relapse preventing. Keywords: diabetic foot, sepsis, metabolism challenge, surgical correction, ileoduodenoplasty.

The paper provides a comparison of properties of cryopreserved fetal murine multipotent stromal cells (MSCs) of skin-muscular origin and those derived from adult thymus in culture in vitro. Fetal MSCs showed a 30% higher number of average population doublings within 24 hrs, and 41% lower average population doubling time. It was found that the fetal MSCs of the 4th passage had a 39% higher clonogenic activity than the adult thymus-derived ones. Fetal MSCs and those derived from adult thymus differentiated in osteogenic and adipogenic lineages with equal efficiency in special culture media. Fetal and thymus-derived MSCs were characterized by almost the same high ability of contact interaction with thymocytes, and the fibroblast-lymphocyte rosette (FLR) formation. They were far less active in FLR formation with lymph node cells. This indicated the presence of membrane affinity for immature lymphoid cells in both MSC subpopulations. The results showed the fetal MSCs to be significantly different from the adult thymus-derived MSCs by more active kinetics of growth and clonogenic potential. However, both cell subpopulations had virtually the same ability for linear differentiation and showed high activity during contact with immature lymphoid cells. Linear differentiation and the ability to interact with lymphocytes were found to be quite stable properties of MSCs, but a proliferative activity and in vitro colony formation distinguished significantly in different types of MSCs. This can be taken into account when choosing the cells for therapy, research and results assessment.