The present study was carried out with the aim to evaluate the relation of the content of proinflammatory and antiinflammatory cytokines with the sperm quality indicators. The study involved 45 patients with a pathological spermogram and 15 practically healthy men. A positive correlation (r = 0.45) between the content of IL-6 and the sperm concentration in the ejaculate and a negative correlation (r =-0.42) of this cytokine with the number of live sperm were revealed. The concentration of IL-8 was positively correlated with sperm density (r = 0.52) and it was negatively correlated with the number of live sperm (r =-0.55). A positive correlation between the content of IL-10 and the sperm concentration as well as the number of active forms was observed (r = 0.45 and 0.5, respectively), and a negative correlation (r =-0.7) was observed between the ejaculate volume and the concentration of IL-10. In the obtained ejaculates, a close positive correlation between the content of IL-8 and IL-6 (r = 0.74) and between the concentration of IL-10 and IL-6 (r = 0.72) was observed. Based on the completed study, a conclusion was made that the increased level of IL-6 and IL-8 may result in the reduction of the sperm quality, and, possibly, infertility, and that IL-10 plays an important role in improving sperm parameters.

To substantiate the possible relationship of the parameters of homeostasis of the concentration of Ca2+, K+, Na+ and the mass of organic and inorganic substances secreted synthesized еt by the tissues of the genital system of males and females (bull – sperm plasma; cow – vaginal mucus), with passive and/or by active methods of movement of sperm and ova through their channels and ducts, the peculiarities of the distribution of components of open and closed systems of the type "environment – cell (substance)" were investigated. Methods of spectrophotometric (flame photometry, Flapho-4) and gravimetric (combustion, extraction, elimination) analysis of the studied samples experimentally established that the adaptive response of sperm to the protective and/or harmful effects of extreme conditions (concentration, temperature, properties of protective environment components). Sperm preservation (TCS) is different. Depending on those (exo-) or other (endogenous) circumstances, the antiportic way of moving ions can change to symportic. The peculiarities of the parameters of concentration and mass of the components of the studied systems revealed in this connection indicate that the fractions of aqueous extracts (PVE) of tissues and secretions of bull and cow, at different functional states of genitals (norm, pathology), have different concentrations of free of bound alkali metal ions, mass and content of substances of their dry residue eliminated in distilled water and insoluble (water, acids). The limit of the difference of the ratio of the ratio (IC:1) of the concentration of one- (Ca2+:Ca2+, K+:K+, Na+:Nа+) and different (Na+:Ca2+, K+:Ca2+, Na+:K+) pairs of ions indicates that that the limit of osmotic pressure parameters of the organic and inorganic components of samples of tissues of genitals, sperm and uterine-vaginal mucus can also be different. This suggests that different parameters of osmotic pressure of the components of the synthesized secretions and the formed sperm and eggs create (initiate, stimulate, inhibit) favorable conditions for their passive and/or active movement through the channels and ducts of the genitals. However, it should be added that the proposed hypothesis requires discussion and experimental confirmation.

The primary aim of the work was to study the peculiarities of the influence of endogenous factors (products of inflammatory processes occurring in the tissues of female genitals) in the biological system of type “environment (uterine-vaginal mucus) – substance (water, organic and inorganic substances)” on the indicators of mass (g, mg) of its components. The results of the experiments indicate that their harmful effect changes the color and density of mucus released during estrus (UVM); reduces the parameters of the mass content of H2O and inorganic substances (IS), but increases – organic (OS). Under these circumstances, the distribution of the parameters of the mass of the components of the dry residue (DR, dried at 105 oC) of UVM of cows of the experimental group is presented in a row where the mass of ОS1 (burn at 520–530 oC) ˃IS ˃ОS2 (burn at 650 оС), but for control is another as well as mass of IS˃ОS1˃ОS2. This means that the harmful effects of inflammatory products on the genital tissues of females negatively affect the distribution of the mass of OS and IS in the system “environment-substance”.