In order to develop an in vitro propagation process for production and conservation, in this study, we investigated the influence of disinfectants and plants growth regulators (PGR) on the infection rate of explants, the ability of callus formation, and the regeneration of shoot and root from Asparagus officinalis L. trunk segments. We obtained the best results when disinfecting trunk segments explants combined with alcohol 70° in 5 minutes and NaClO in 5 minutes with the lowest contamination rate of 3.7%. Callus was formed well in MS medium supplemented with 3% sucrose; 0.7% agar, pH= 5.8; 1.0 mg/l BAP and combined with 0.5 mg/l NAA, 1.5 mg/l BAP and 0.75 mg/l NAA. The highest rate of shoot formation was 87.2% when the callus continued to grow in the environment supplemented with 2.0 mg/l BAP and 1.0 mg/l NAA. Roots were formed after 4 weeks of shoot cultivation in MS medium supplemented with 0.5 mg/l NAA with the highest rooting rate of 74.59%.

Use of raceme’s holders for tomatoes of cocktail type was a positive effect on the number of fruits, their weight and the number of ripening fruits. To determine the significance of the differences, we used a statistical method for comparing the arithmetic means of two samples. It has been established that the weight of the fruits with raceme holders (343.7 g) differs from the weight without raceme holders (317.0 g). The difference in weight is 7.7%. The share of ripe fruits of plants with raceme holders was 93%, and without raceme holders -89%.