Objectives: As very few studies were done on the freezability of pure Egyptian cattle bull sperm. So, we designed this study to evaluate the individual variations in freezability of native bull semen extended in Tris based diluent and sodium citrate-based diluent. Methods:Semen was collected by artificial vagina, examined at once in farm laboratory. Only semen samples fit the minimum parameters are extended in two extenders first the universal one (TRIS) and second modified Sodium citrate extender by adding glycerol (CU-16) which created at Cornell University to evaluate the individual bull variation and interaction between extender and bull.Results: Post-Thawing individual sperm motility, live, abnormal, acrosome integrity percentage was evaluated in addition to Hypo-Osmotic Swelling test (HOS). The highest value for motility, live abnormal and acrosome percentage were 44.00 ± 1.12, 52.25 ± 1.60, 21.25 ± 0.81 and 62.80 ± 2.58 from bull 2, 1, 3 and 2, respectively for semen extended in TRIS, and 42.25 ± 1.61, 52.00 ± 1.76, 22.15 ± 0.85 and 57.40 ± 3.07, from bull 1, 1, 4, 3, respectively for semen extended in CU-16. The results of HOS were 59.25 ± 1.76 and 55.95 ± 2.13 from bull 1 extended in TRIS and CU-16, respectively. Conclusion:A significant variation (p<0.05) in tested parameter was clear when semen extended in TRIS extender with the absence of such variation when semen extended in CU-16 except in live sperm percentage. With ignoring the extender effect a clear significant variations were detected between bulls in all tested parameters.
Background: The placenta is a life-supporting organ for the growing fetus. Several studies have highlighted the relationship between placental weight and neonatal birth weight; however, the role of glycosylated hemoglobin (HbA1c) has not been addressed. Aim: The study aimed to determine the relationship between the placental weight, neonatal birth weight, and HbA1c at term pregnancy in a Sudanese hospital. Methods: This was a cross-sectional hospital-based study conducted at Al Ban-Jaded Hospital between November and December 2022. Data, including the gestational age at delivery (in weeks), parity, mode of delivery, fetal birth weight, placental weight, fetal gender, and presence or absence of maternal medical diseases, was obtained from 60 singleton term deliveries who met the inclusion criteria for the study. The tested data was analyzed using SPSS version 25, and t-test was used to compare the statistical significance (P value ≤ 0.05 was considered statistically significant). Results: The mean age was 27.02 ± 6.8 years. The mean HbA1C was 5.98% ± 0.39% (5.2%–6.9%). Glycated hemoglobin was elevated (≥5.7%) in the majority (46, 76.7%) of pregnancies. The mean placental weight was 290 ± 106 g (100 g–700 g), while the mean birth weight was 3.00 kg ± 0.36 kg (2.3 kg–3.8 kg), and the placental/birth weight ratio was 9.6%. There was a significant positive correlation between neonatal weight and placental weight (r=0.514, P value=0.000). Also, neonatal weight and placental weight had a significant positive correlation with HbA1C (r=0.657, P value=0.000). Placental weight and neonatal birth weight significantly increased with the gestational age of neonates (P value <0.05), with a slight drop noted in term gestation. Conclusions: There is a positive correlation between placental weight and birth weight of the neonate in pregnancies with high maternal HbA1C, and placental weight and birth weight were higher than in pregnancies with normal maternal HbA1C.
Background: The placenta is a life-supporting organ for the growing fetus. Several studies have highlighted the relationship between placental weight and neonatal birth weight; however, the role of glycosylated hemoglobin (HbA1c) has not been addressed. Aim: The study aimed to determine the relationship between the placental weight, neonatal birth weight, and HbA1c at term pregnancy in a Sudanese hospital. Methods: This was a cross-sectional hospital-based study conducted at Al Ban-Jaded Hospital between November and December 2022. Data, including the gestational age at delivery (in weeks), parity, mode of delivery, fetal birth weight, placental weight, fetal gender, and presence or absence of maternal medical diseases, was obtained from 60 singleton term deliveries who met the inclusion criteria for the study. The tested data was analyzed using SPSS version 25, and t-test was used to compare the statistical significance (P value ≤ 0.05 was considered statistically significant). Results: The mean age was 27.02 ± 6.8 years. The mean HbA1C was 5.98% ± 0.39% (5.2%–6.9%). Glycated hemoglobin was elevated (≥5.7%) in the majority (46, 76.7%) of pregnancies. The mean placental weight was 290 ± 106 g (100 g–700 g), while the mean birth weight was 3.00 kg ± 0.36 kg (2.3 kg–3.8 kg), and the placental/birth weight ratio was 9.6%. There was a significant positive correlation between neonatal weight and placental weight (r=0.514, P value=0.000). Also, neonatal weight and placental weight had a significant positive correlation with HbA1C (r=0.657, P value=0.000). Placental weight and neonatal birth weight significantly increased with the gestational age of neonates (P value <0.05), with a slight drop noted in term gestation. Conclusions: There is a positive correlation between placental weight and birth weight of the neonate in pregnancies with high maternal HbA1C, and placental weight and birth weight were higher than in pregnancies with normal maternal HbA1C.
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