Methyl bromide is an excellent fumigant but has been banned because it has high potential for depleting the ozone layer which leads to many environmental and human health hazard issues. In this connection, effectiveness of carbon dioxide (CO, 99.9%) was studied as an alternative to methyl bromide under various exposure timings, 25 ± 1 °C, against different developmental stages of the almond moth, Cadra cautella, and red flour beetle, Tribolium castaneum. In case of C. cautella, the LT against adult, pupa, and larval stages was achieved after 37.5, 78.1, and 99.9 h of CO application, respectively. While for T. castaneum, the LT values were obtained after exposure timings of 29.3, 153.9, and 78.4 h against adult, pupa, and larval stages, respectively. Adults were very susceptible; in contrast, pupae and larvae were more tolerant. The susceptibility order was observed as follows: T. castaneum adult > C. cautella adult > C. cautella pupae > T. castaneum larvae > C. cautella larvae > T. castaneum pupae. This study could be useful in developing the management strategies to prevent stored dates from C. cautella and T. castaneum infestation.

The almond moth, Cadra cautella (Walker) (Lepidoptera: Pyralidae), is a serious worldwide pest of dates and other crops, both in the field and in storage. Infestation by this pest significantly limits the sale and export of dates. Modified atmospheres, the temperature manipulations, are the best eco-friendly alternatives in stored product protection. We examined temperature regulation as an ecologically friendly method of pest management in stored food commodities, in particular, its effects on characteristics of C. cautella biology, including development time, oviposition period, fecundity, fertility, and longevity of adults reared on date cultivar 'Khodari,' at temperatures of 15, 25, and 35 ± 1 °C, with 65 ± 5% relative humidity and a photoperiod of 15:9 (L:D) h. Six larval instars were recorded at each temperature. Larval development was sluggish at 15 °C; therefore, daily observations were terminated after 180 d of the larval span for this temperature. Total larval spans of 48.95 ± 0.76 and 32.12 ± 1.08 d were recorded at 25 °C and 35 °C, respectively. A mean ovipositional period of 5.80 ± 0.44 d yielding 213.60 ± 13.41 eggs per female, with hatchability of 85.94 ± 2.97%, was recorded at 25 °C, whereas at 35 °C, a mean ovipositional period of 1.60 ± 0.26 d yielded 19.80 ± 5.11 eggs per female, with no hatchability. These differences were highly significant. Our observations of poor development at 15 °C, lower numbers of eggs and absence of hatchability at 35 °C, together with head capsule measurements and growth ratios, can be of benefit when planning management strategies to reduce C. cautella infestation.

Date palm, is a tree of economic importance which is grown around the world, including Saudi Arabia. Its fruit is nutritious and possesses medicinal benefits. Almond moth, is a serious date fruits pest in the field as well as in the storage and causes severe economic losses. In the given research, ultraviolet radiation type B (UV-B, 315 nm) harmful effects were evaluated against all developmental stages of C. cautella. One and 3-d-old eggs, 12 and 18-d-old larvae, 1-d and 6-d-old pupae, and 1-d-old adults, were exposed to UV-B for different intervals. Eggs were exposed for 0–30 min and 0% hatchability was achieved both for 1-d and 3-d-old eggs after 30 min. The larvae were exposed for 6–24 h, and after 24 h, mortality was 100 and 97% for 12 and 18-d-old larvae, respectively. Similarly, the pupae were exposed for 0–30 h, and 100% mortality was achieved after 30 h for 1-d-old pupae. Furthermore, none of the 6-d-old pupae emerged as an adult after 12 h of exposure. When adults were exposed for 1–4 d, no mortality was observed; however, UV-B reduced fecundity and hatchability in the treated adults. The susceptibility order was as follows: eggs > larvae > pupae > adults. Several uncharacteristic behaviors of C. cautella were noted, such as females depositing eggs openly on food items and containers, mature larvae exiting from food, larvae starting to wander for pupation, and pupation occurring typically outside the food. The application of UV-B could be an effective management strategy because all developmental stages of C. cautella were susceptible to UV-B that might be helpful to protect the dates from C. cautella infestation.

Vitellogenins, major yolk protein precursors, play an essential role in the reproduction and spread of all oviparous species, including insects. To investigate reproductive strategies of the warehouse moth Cadra cautella at the molecular level, a partial transcript of the C. cautella vitellogenin (CcVg) gene was extended through the rapid amplification of cDNA ends PCR and sequenced. The complete CcVg mRNA transcript was 5,334 bp long, which encoded a protein of 1,778 amino acids, including the first 14 amino acids of the signal peptide. The deduced CcVg protein contained a putative cleavage site (RTRR) at the amino-terminal side, similar to several other insect species. DGQR and GI/LCG motifs were present at the CcVg gene C-terminus, followed by nine cysteine residues. CcVg harbored 131 putative phosphorylation sites, numbering 84, 19, and 28 sites for serine, threonine, and tyrosine, respectively. The transcript showed a great resemblance with other lepidopteran Vgs. CcVg protein analysis revealed three conserved regions: 1) vitellogenin-N domain, 2) DUF 1943 (domain of unknown function), and 3) a von Willebrand factor type D domain. Additionally, sex, stage-specific, and developmental expression profiles of the CcVg gene were determined through RT-PCR. The Vg was first expressed in 22-day-old female larvae, and its expression increased with growth. The phylogenetic analysis based on different insect Vgs revealed that the CcVg exhibited close ancestry with lepidopterans. The CcVg-based RNAi experiments were performed, and the effects were critically evaluated. The qRT-PCR results showed that CcVg-based dsRNA suppressed the Vg gene expression up to 90% at 48 h post-injection. Moreover, CcVg-based RNAi effects resulted in low fecundity and egg hatchability in the CcVg-based dsRNA-treated females. The females laid eggs, but because of insufficient yolk protein availability the eggs could not succeed to hatch. The significant difference in the fecundity and hatchability unveils the importance of CcVg gene silencing and confirmed that the Vg gene plays a key role in C. cautella reproduction and it has the potential to be used as a target for RNAi-mediated control of this warehouse pest.

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