Needle Stick Injury or NSI is a term for occupational accidents experienced by health workers caused by needling or sharp medical objects that have been contaminated with infectious fluid from a patient. Potential transmission of disease infections can occur due to needles / sharp used medical items used for patients who injure the exposed staff. One of the preventions of Needle Stick Injury is by providing education to improve nurses' understanding and attitude in universal precautions. This study aims to determine the effect of offering education on nurses' knowledge and attitudes About Needle Stick Injury (NSI) in applying universal precautions in Private Hospitals -Central Jakarta. The research design used is a quasi-experiment, with a Pre Test and Post Test without Control design approach. The population in this study were nurses with a sample of 107 people. The sampling technique uses total sampling. The study's results using the Wilcoxon Sign Test show no differences in the level of understanding of nurses before and after being given education with p-value = 0.220 (> 0.05). While the nurse attitude variable using Paired T-test showed the differences in nurses' attitudes before and after being given education with P-value = 0,0001. Based on these results, education can be used to improve nurses' attitudes in the application of universal precautions.