To gain greater understanding of the role of Streptococcus mutans and Veillonella in the caries process, studies of both aerobically and anaerobically grown plaques of S. mutans C67-1 and V. alcalescens V-1 on human enamel slabs were carried out in an artificial mouth. Plaque development, acid production, and demineralization were measured. Early plaque development of monobacterial and mixed bacterial plaques started from randomly adhering cells on day 1 to confluent multilayered microcolonies on day 4. Differences were observed in viable cell counts, total cell mass, and in acid production. In most cases CFU, DNA and acid production were higher in the mixed bacterial plaque, especially in the anaerobic mixed plaque. Lactic acid was the predominant acid in all cases following the supply of sucrose to the plaque. No decisive role could be found for acetic, formic, and propionic acid. No inhibition of demineralization was observed in the enamel slabs inoculated with both aerobic and anaerobic mixed plaques. Demineralization ranged from the more classical picture of lesion development in the aerobic monobacterial plaque-treated samples to an aggressive etching of the enamel surface in the anaerobically mixed treated slabs.
A new apparatus for the continuous cultivation of mono and mixed bacterial plaque on solid surfaces is described. The features are: easy preparation and handling; freedom from technical problems and microbial contamination; self-sufficient for periods of up to 56 d; 12 samples are taken simultaneously; programmable supply inlet. Experiments were performed with Streptococcus mutans C 67-1 for mono bacterial inoculation and in combination with Veillonella alcalescens V-1 for mixed bacterial inoculations. The results showed that the controlled conditions and versatility of the apparatus make possible the study of plaque-development and lesion production on a time-dependent basis. It is concluded that the apparatus is suitable for a wide range of dental and non-dental applications.
With the absence or loss of an ear due to a congenital disorder, trauma, or malignant disease, an auricular prosthesis can be provided. In some patients, surgical ear reconstruction is an option, but this may be difficult, especially in burn victims or after oncological resections followed by radiotherapy. Even when 3D technology is used to fabricate a mold, the esthetic results are often disappointing. [1][2][3] Therefore, in most situations, silicone auricular prostheses are provided.Auricular prostheses can be retained with skin adhesive or with extraoral implants. Although implant retention is generally preferred, the choice between adhesive and implants depends on technical and patient-related factors. 4,5 Skin adhesives have disadvantages. Placing the prosthesis in the correct position can be difficult, the adhesives can dissolve leading to loss of retention, the skin, especially after radiation therapy, can become irritated, and skin reactions can result from intolerance to components of the adhesive or the solvent. 5
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