Tooth discoloration due to intra coronal bleeding after trauma can cause aesthetic disturbances and appearance. Internal bleaching is a non-invasive method to restore tooth color which is performed after endodontic treatment by placing a strong oxidizing agent in the pulp chamber. Aim of this study was to report the success of internal bleaching procedure to restore discolored teeth due to trauma. A 44-year-old female came to Conservative Dentistry Universitas Airlangga with chief complaint of the upper right insisor tooth looks darker than the adjacent teeth. The patient had fallen 10 years ago from her motorcycle and her tooth had been painful and then the pain disappeared. In intra oral clinical examination, it showed that tooth 11 had discoloured, vitality test of tooth 11 showed no response to electric pulp test. Radiographic view showed that radiolucen in periapical area and no fracture was found. At the first visit, treatment of single visit root canal using rotary instrument and gave dressing calcium hydroxide. A week later on the second visit, internal bleaching with hydrogen peroxide 35% was done. After bleaching, calcium hydroxide was applied to neutralize the oxidizing agent and then re-evaluated for the color. At the third visit, it showed that the tooth colour was obtain as desire from C3 to A3 (Vitapan Classical). The internal bleaching procedure on the traumatized tooth was the best option to restore the function and aesthetics according to the original tooth colour and also provided benefits in terms of saving time, lower price, and maximum results.

This research aimed to determine whether irradiation of 405 nm diode laser with chlorophyll as photosensitizer could degrade the extracellular polymeric substance (EPS) of Enterococcus faecalis (E. faecalis) biofilm. The material for this study needs 25 biofilm formed by E. faecalis was divided equally into five groups. The control negative group (C-) consisted of E. faecalis biofilm, the control positive group (C+) consisted of E. faecalis biofilm and chlorophyll photosensitizers, and the other three treatment group (T1, T2, T3) consisted of E. faecalis biofilm and chlorophyll photosensitizers. Each treatment groups were irradiated for 90 second (s) for T1 group, 105 s for T2 group, and 120 s for T3 group with 405nm diode laser. The degradation EPS of E. faecalis’ biofilm was determined using Confocal Laser Scanning Microscope (CLSM). Irradiation duration affected the degradation EPS of E. faecalis’ biofilm. Chlorophyll with 120 s laser irradiation showed significant degradation EPS of E. faecalis’ biofilm compared to other groups (p < 0.05). Irradiation of diode laser 405nm with chlorophyll photosensitizer 120 s could degrade EPS of E. faecalis biofilm up to 97.51%.

Adhesive dentistry’s main assumption is to create a strong chemical bond between dental hard tissues and restorative composite material. One of the most important aspects of this interface is the hybrid layer. Unfortunately, due to physical and chemical causes, the hybrid layer wears away with time. Epigallocatechin-3-gallate (EGCG), a component extracted from green tea, has several roles in the medical and dentistry field including as a crosslinking agent and as a chelating agent. Although there are several negative results, EGCG was proven to be able to preserve resin-dentin bonds without harming the restoration. As a crosslinking agent and chelating agent, EGCG has the potential to enhance the physical properties of dentin collagen and resin-dentin adhesion. The purpose of this study was to see how EGCG, as a cross-linking agent, affected dentinal collagen and hybrid layers, as well as how chelating chemicals affected Matrix metalloproteinase (MMPs).