ABSTRAK Kali Wiso merupakan sungai yang berada di tengah kota Jepara. Perairan ini menjadi tempat pembuangan limbah-limbah secara langsung. Limbah tersebut diantaranya limbah domestik, limbah pasar, limbah kapal, serta limbah TPI. Berdasarkan masukan limbah tersebut menjadikan muara ini tercemar. Perairan yang tercemar dapat dilihat dari pengamatan secara fisika, kimia, maupun biologis. Kondisi perairan yang tercemar secara biologis dilihat dari keberadaan bakteri patogen yang ada di perairan. Indikator bakteri yang digunakan yaitu bakteri coliform, karena sifatnya yang berkorelasi positif dengan bakteri patogen lainnya. Pemanfaatan perairan ini digunakan untuk kegiatan pelabuhan, tempat bersandar kapal nelayan, serta kegiatan perikanan yang ada di sekitar perairan Jepara. Oleh karena itu perlu diketahui kepadatan bakteri coliform sehingga dapat bermanfaat sesuai dengan peruntukannya. Tujuan dari penelitian ini adalah untuk mengetahui total bakteri coliform serta mengetahui adanya bakteri Escherichia coli. Penelitain ini dilakukan pada bulan Maret 2016 di Muara Kali Wiso dengan dua kali pengulangan dalam kondisi pasang dan surut. Metode yang digunakan yaitu survei dengan teknik sampling purposive sampling. Metode analisa laboratorium yang digunakan berdasarkan SNI -01-2332-1991. Kepadatan bakteri coliform pada perairan muara Kali Wiso yaitu >110.000 sel/100ml dan bakteri Escherichia coli sebesar >110.000 sel/100ml. Pada kondisi pasang dan surut kepadatan bakteri coliform dan Escherichia coli memiliki nilai perikaraan yang sama, namun tidak menandakan bahwa total bakteri keduanya sama. Kepadatan bakteri coliform dan Escherichia coli telah melebihi batas kriteria mutu air yang telah ditetapkan. Keberadaan bakteri patogen ini bisa mengkontaminasi biota-biota yang ada di perairan. Sehingga jika biota tersebut dikonsumsi oleh manusia bisa menyebabkan gangguan kesehatan secara tidak langsung. Kata kunci: Muara Kali Wiso; Bakteri Coliform; Bakteri Escherichia coli ABSTRACT Kali Wiso is the river in the middle of Jepara. This river receives wastes disposal from surrounding across. The waste including domestic waste, market waste, ship waste, and waste from fish market. Based on the inputs of the waste that made the estuary polluted. Polluted waters can be seen from the observation of physical, chemical, and biological. The conditions of the waters which biologically polluted are recognized from the pathogenic bacteria existing in these waters. The indicator of bacteria used, namely coliform bacteria, because of its positive correlation with other pathogenic bacteria. The utilization of these waters is used for the activities of the port, fishing pout, and fishing activities in the waters around Jepara. Therefore, its important to know the density of coliform bacteria so that can be advantageous according to its purpose. The purpose of this study to determine total of coliform bacteria and the existence of Escherichia coli bacteria. This research conducted in March 2016 at Kali Wiso estuary with on the condition of ups and downs with two repetitions. The method used is a survey with purposive sampling technique. Laboratory analysis method used by ISO -01-2332-1991. The density of coliform bacteria in the waters of the Kali Wiso estuary is >110.000 cells/100ml and Escherichia coli bacteria is >110.000 cells/100ml. On the condition of ups and downs density of coliform bacteria and Escherichia coli have the same approximate value, but it does’nt signify that the total of bacteria both are the same. The density of coliform bacteria and Escherichia coli have exceeded the water quality criteria that have been set. The existence of these pathogenic bacteria can contaminate the biota in aquatic. Therefore, this biotics are consumed by humans, it can cause health problem indirectly. Keywords: Kali Wiso Estuary; Coliform Bacteria; Escherichia coli Bacteria

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Fertilization was doing to measure the success of sperm cryopreservation because it was more informative. Sperm cryopreservation was expected to get sperm that successfully fertilize the egg well. The aim of this study was to determine the sperm fertilization ability of giant grouper (Epinephelus lanceolatus, Bloch 1790) 48 hours post-cryopreservation in fertilizing tiger grouper eggs (Epinephelus fuscoguttatus, Forskal 1775). Fertilization was carried out artificially using cryopreservation sperm with 6% of glycerol and palm dates concentrates (0%; 5%; 10%; 15%; 20%; and 25%). The tiger grouper brood was previously injected with hormone chorionic gonadotropin at a dose of 500 IU/kg. The results showed that there were significant differences (P < 0.05) on fertilization of tiger grouper eggs with sperm of giant grouper which were cryopreserved with 6% of glycerol and various concentrations of palm date concentrates. Sperm which was cryopreserved with 6% of glycerol and 10% of palm date concentrate has the best fertility ability by showing the highest value of 66.25 ± 3.23%.