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Abstract. E-learning is becoming more and more popular, which is a kind of learning mode based on network, with the dramatic development of computer and network technology. Although e-learning system is bringing about an education revolution, the lack of emotion and interactivity always persecute students and researchers. In this paper, a system architecture based on e-learning is proposed, which focused on interaction. In order to prompt students' experience of learning, we analyze the relationship of students' emotion and teaching content based on Kansei Engineering. A novel approach is proposed to evaluate students' emotion to teaching content, which can improve students' learning efficiency.

<p>Supplementary Table S1: Clinicopathological features of osteosarcoma patients Supplementary Table S2: Primer sequences used for Quantitative PCR Supplementary Table S3: Primers used for ChIP experiments Supplementary Table S4: Tumor forming ability following subcutaneous injections</p>