Abstract. We here report a novel biomimetic mineralization strategy for enamel remineralization by intergration of calcium phosphate loaded and thermally triggered liposomes and a self-assembly amelogenin-inspired peptide. Firstly, calcium and phosphate loaded temperature sensitive liposomes were synthesized by Interdigitation-fusion method with 1,2-bis(palmitoyl)-sn-glycero-3-phosphorcholine (DPPC) and 1,2-bis(myristoyl)-sn-glycero-3-phosphocholine (DMPC) at mass ratio of 9:1. The liposomes were stable at room temperature, but slowly released calcium and phosphate ions if heated to 37°C . Secondly, a novel polyanion amelogenin-inspired oligopeptide (Gln-Pro-Ala) 4 -Thr-Lys-Arg-Glu-Glu-Val-Asp) was synthesized by standard solid-phase. Finally, the mixture of peptide and liposomes solution was exposed to enamel surface at 37°C . The results showed oriented enamel-like hydroxylapatite evenly deposited on enamel surface.

Minocycline, a semi-synthetic tetracycline antibiotic, was incorporated into gelatin- hydroxyapatite (HA) composite by using a biomimetic co-precipitation method, Firstly, a certain amount of acidic solution of hydroxyapatite was added into gelatin solution dropwise. After that, different amounts of minocycline (0mg, 50mg, 100mg, 200mg, 300mg) solution was added into the reaction system respectively , and obtain the hydroxyapatite - gelatin- minocycline composite. The results showed that the amount of the minocycline impacted the structure of the composite, and minocycline affects HA crystal growth by maybe bonding to 300 face. The approach described here may provide a basis for the preparation of an antibacterial biomaterial for bone regeneration.