In this paper, the immune adsorbents were prepared for adsorption of aflatoxin M1 in milk. The copolymerization of glyceryl methacrylate (GMA) and methyl methacrylate (MMA) microspheres were prepared using dispersion polymerization with azodiisobutyronitrile (AIBN) as initiator in ethanol-aqueous medium in the presence of polyvinylpyrrolidone (PVP) as dispersant under various conditions. Using epoxy groups on the microsphere surface AFM1 monoclonal antibody were coupled on the microspheres .The adsorption quantity of monoclonal antibodies for aflatoxin M1 in the milk was more than 1.2 µg/g. The results showed that the selective adsorption for aflatoxin M1 in milk of immune adsorbent is good. It was an ideal immune adsorption material for removed of aflatoxin M1 in milk.
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