Simple, cost effective, high sensitive and selective detection strategies for disease related DNA sequences in clinical diagnostics and research purposes are still on demand. Detection of DNA specific sequences of particular biomedical importance, based on electrochemical signaling, has been reported as a promising analytical approach for medical diagnostics due to its simplicity, cost effective, sensitivity, selectivity and rapidity. Herein, a simple and cost effective DNA biosensor based on poly T30 Copper Nanoparticle Signaling and biobarcode technique is presented for the first time. In this design, complementary sequence places the poly T30 modified bio-barcode probe (P2-SiO2-T30) on the sensor interface. Upon copper reduction reaction, copper nanoparticles (CuNPs) are clustered along the poly T30 modified bio-barcode probe (P2-SiO2-T30-CuNPs). During electrochemical measurements copper nanoparticles (CuNPs) are oxidized to give current signal. This detection strategy has a detection limit of 10 pM.