A novel polysaccharide structure (PS-T80) was collected from Ophiocordyceps sobolifera biomass and characterized via a combination of chemical and spectral analyses. Employing high-performance gel permeation chromatography (HPGPC), the average molecular weight is proven to be 7.4 × 10 4 Da. Furthermore, a sugar composition analysis of the obtained polysaccharide suggests two main sugars, β- d -glucose and α- d -mannose, at a molar ratio of 2:1, respectively, in the backbone. The structure analysis unveils that PS-T80 is a mannoglucan, possessing the repeating unit of [→3)-β- d -Glcp-(1 → 3)-α- d -Manp-(1 → 3)-β- d -Glcp-(1→] n . Such a configuration could be considered a novel polysaccharide. Impressively, in vitro antioxidant tests revealed that PS-T80 has a promising antioxidant activity. These results demonstrate that the obtained PS is a potential bioactive material for biomedical applications.

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The presented study attempts to unveil and evaluate the antioxidant activity of a novel heteropolysaccharide separated from the roots of Myxopyrum smilacifolium (denoted as PS-MSR). The molecular weight of PS-MSR is found to be 1.88 × 104 Da and contains two principal sugars, which are d-glucose and d-fructose, in the backbone. Decoding the structure of the obtained PS-MSR sample has disclosed a novel polysaccharide for the first time. Indeed, the PS-MSR is composed of (1 → 3)-linked glucosyl units and (2 → 3)-linked fructosyl units. In addition, the 1D and 2D NMR spectra of the PS-MSR sample display the repeating unit of the isolated polysaccharide, [→3)-α-d-Glcp-(1 → 3)-β-d-Frucf-(2 → 3)-β-d-Frucf-2 → 3)-)-β-d-Frucf-β-(2→] n . Interestingly, the PS-MSR sample exhibits outstanding antioxidant activity, signifying the potential utilization of the explored polysaccharide for antioxidant-based material.

Nowadays, the use of mushrooms in medicine is ubiquitous and has achieved particular success. The antioxidants in mushrooms can deactivate free radicals. This study assesses the antioxidant potential of mushroom Calocybe indica with the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging methods and the total antioxidant capacity. The mushroom’s ethanol extract exhibits acceptable activity with a low IC50 value (240.11 μg/mL), approximately 2.9 times lower than that of the mushroom Ophiocordyceps sobolifera extract. The ABTS scavenging rate of the extract is around 60% at 500 µg/mL, and the total antioxidant capacity is equivalent to 64.94 ± 1.03 mg of GA/g or 77.42 ± 0.42 μmol of AS/g.  The total phenolics, flavonoids, polysaccharides, and triterpenoids are equivalent to 29.33 ± 0.16 mg of GAE/g, 17.84 ± 0.11 mg of QUE/g (5.04 ± 0.04%), and 4.96 ± 0.04 mg of oleanolic acid/g, respectively. Specifically, the total triterpenoid content has been reported for the first time. The mushroom can have potential biomedical applications.

The presented investigation attempts to offer a robust, simple, cost-effective AgNPs/MnO 2 /ErGO composite to simultaneously determine piroxicam (PRX) and ofloxacin (OFX). The material is prepared via facile depositions of MnO 2 and Ag species on graphene oxide followed by an electroreduction. The achieved composite (i.e., AgNPs/MnO 2 /ErGO) exhibits intimate interfacial contacts, promoting charge transfer and mass transport. Consequently, the sample AgNPs/MnO 2 /ErGO-decorated glassy carbon (GC) electrode (denoted AgNPs/MnO 2 /ErGO-GCE) signifies a remarkable capability to monitor PRX and OFX using differential pulse adsorptive anodic stripping voltammetry (DP-AdASV) technique. Indeed, the sensitivity values (MLD) have been found to be 0.063 and 0.077 μM for PRX and OFX, respectively, while maintaining high sensitivity, selectivity, repeatability, and stability. Impressively, the obtained electrode endows a remarkable performance in the determination of real antibiotic and water samples, which are compatible with the HPLC analysis, paving the way toward a high-performance electrochemical sensor for antibiotics.