Bamboos, regarded as therapeutic agents in ethnomedicine, have been used to inhibit inflammation and enhance natural immunity for a long time in Asia, and there are many bamboo associated fungi with medical and edible value. In the present study, a total of 350 fungal strains were isolated from the uncommon moso bamboo (Phyllostachys edulis) seeds for the first time. The molecular diversity of these endophytic fungi was investigated and bioactive compound producers were screened for the first time. All the fungal endophytes were categorized into 69 morphotypes according to culturable characteristics and their internal transcriber spacer (ITS) regions were analyzed by BLAST search with the NCBI database. The fungal isolates showed high diversity and were divided in Ascomycota (98.0%) and Basidiomycota (2.0%), including at least 19 genera in nine orders. Four particular genera were considered to be newly recorded bambusicolous fungi, including Leptosphaerulina, Simplicillium, Sebacina and an unknown genus in Basidiomycetes. Furthermore, inhibitory effects against clinical pathogens and phytopathogens were screened preliminarily and strains B09 (Cladosporium sp.), B34 (Curvularia sp.), B35 (undefined genus 1), B38 (Penicillium sp.) and zzz816 (Shiraia sp.) displayed broad-spectrum activity against clinical bacteria and yeasts by the agar diffusion method. The crude extracts of isolates B09, B34, B35, B38 and zzz816 under submerged fermentation, also demonstrated various levels of bioactivities against bambusicolous pathogenic fungi. This study is the first report on the antimicrobial activity of endophytic fungi associated with moso bamboo seeds, and the results show that they could be exploited as a potential source of bioactive compounds and plant defense activators. In addition, it is the first time that strains of Shiraia sp. have been isolated and cultured from moso bamboo seeds, and one of them (zzz816) could produce hypocrellin A at high yield, which is significantly different from the other strains published.

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Micro and nanoscale 3D printing technic is applied to fabricate functional insulating material which mitigates surface discharge in vacuum based on the microscopic electron multipactor suppression. The proposed alumina ceramic insulator design consists surface-embedded thin metal wires which introduce a local gradient of secondary electron emission yield, such that the trajectories of multipactor electrons are distorted by accumulated negative surface charges and the secondary electron emission avalanche across the insulator surface is intermitted. Considerable increases of surface flashover threshold and surface charging reduction are verified by experiment. Also, additional efforts are made to determine the optimal size and spatial distribution of the metal wire. A convex-shape flashover voltage trace is observed when increasing the wire width, suggesting a trade-off between the multipactor mitigation and the insulator strength. Wire position between the adjacency of cathode triple junction and middle of the insulator is proved to be favorable for flashover mitigation. The physical details of surface flashover mitigation by the proposed insulator design are revealed by an ab initio particle-in-cell (PIC) simulation code, corroborating the experiment from microscopic aspect.

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