1. This study was conducted to determine the effects of enzyme supplementation of maize/wheat-based diets on the performance, egg quality, and serum and bone parameters of laying hens. 2. During the 12-week experimental period, a total of 72 laying hens aged 52 weeks were randomly distributed among 6 experimental groups. Each experimental group contained 4 replicates, each with three birds. The experiment was a randomised design consisting of a 3 × 2 factorial arrangement, with three levels of wheat substitution and two levels of enzyme (xylanase: 1500.00 U/kg, β-glucanase: 100 000 U/kg, cellulase: 1 000 000 U/kg, α-amylase: 160 000 U/kg) inclusion in the diet. Wheat replaced 0, 50, or 100% of maize with or without 1.0 g/kg enzyme supplementation in iso-nitrogenous and iso-caloric experimental diets. 3. Body weight, egg production, egg weight, egg mass, eggshell thickness, and the feed conversion ratio were adversely affected by the wheat-based diet. The eggshell quality parameters decreased with enzyme supplementation to the diet. 4. Wheat-based diets adversely affected calcium and phosphorus concentrations in the tibia, but the addition of the enzymes to the wheat-based diet prevented the negative effects of wheat-based diets on tibia mineralisation in laying hens. The wheat-based diets tended to reduce plasma mineral contents, and the addition of enzymes tended to affect plasma minerals and biomechanical properties of the tibia positively in laying hens. 5. These results indicate that wheat-based diets in aged laying hens adversely affected the mineral metabolism compared with maize-based diets, and the negative effects of wheat on bone mineralisation can be prevented by enzyme supplementation to the diets in laying hens.

One of the factors affecting the success of endodontic treatment is to fill the root canal system hermetically. The aim of this in vitro study was to evaluate the effect of nonthermal plasma (NP) on dentinal tubule penetration of root canal sealers using confocal laser scanning microscopy. Forty mandibular premolar teeth were selected and the root canals were prepared with large‐Waveone‐Gold rotary‐files. Specimens were divided into four experimental groups according to sealer and NP treatment (n = 10). G1: AH‐Plus (AH) G2: nonthermal plasma application + AH‐Plus(AH‐P) G3: Endosequence‐BC(BC) G4: nonthermal plasma application + Endosequence‐BC(BC‐P). Cold lateral‐condensation technique was used for the obturation of root canals. The roots were sectioned horizontally and the sections were examined under confocal laser scanning microscopy. The maximum tubule penetration and percentage of penetration values were obtained from the microscopy images and were statistically analyzed with repeated measurements‐ANOVA and the Tukey (HSD) test (p < 0.05). The percentages of dentinal tubule penetration of the groups were not statistically different. The maximum tubule penetration of the AH‐P was statistically lower than that of the BC‐P (p < 0.05). Plasma application had no affect on the percentage of dentinal tubule penetration. Under the conditions of this in vitro Endosequence‐BC sealer showed higher maximum tubule penetration values than AH‐Plus after NP treatment. Percentage of dentinal tubule penetration values of experimental groups was similar.

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The aim of this study was to determine subclinical mastitis with the help of logistic regression of milk quality determined factors and some features the research material consisted of 204 (145 Holstein, 59 Brown Swiss) dairy cattle raised in a private cattle farm in Konya Province, Turkey. The independent variables considered for the detection of subclinical mastitis are breed, somatic cell number (SCC), color values (L, a, b, H, C), freezing point (FP), pH, electrical conductivity (EC), milking day (MD), lactation order (LO). The dependent variable of logistic regression was CMT score. According to the results of the study, the spescifity was 95.7% and the sensitivity was 57.6%. In general, the predicted value of the accuracy of all data was 83.3%.

The aim of this study was to determine effects of different dietary rosemary essential oil levels on growth performance, carcass traits and haematological values of breeder chukar partridge. Eighty (60 females and 20 males), 32-week-old breeder chukar partridge were randomly divided in five treatments, with four replicate pens with 4 birds each. Five experimental diets were formulated according to rosemary essential oil inclusion rates 0 mg/kg, 24 mg/kg, 48 mg/kg, 72 mg/kg and 96 mg/kg, respectively. The experiment lasted for 8 weeks. The chukar partridges had free access to ad libitum feed and water. At the end of the trial, 1 male and 1 female partridge were slaughtered for blood analysis. The obtained results showed that there was no significant difference in feed intake, body weight gain between the control and treatment groups. Dietary rosemary essential oil supplementation did not affect blood serum glucose, urea, triglyceride, total cholesterol, HDL-C and LDL-C levels in breeder Chukar partridges. It could be concluded that supplementing rosemary essential oil into Chukar partridges diet show no negative effect on growth performance.