Eleutherine palmifolia (L.) Merr. (E. palmifolia or Dayak Onion) is an anticancer plant used for traditional medicines. The difference of cultivation sites may affect metabolites content, pharmacological activity and toxicity profiles. This study aimed to determine the metabolite fingerprinting, anticancer and toxicity profiles of E. palmifolia from several regions in Indonesia for authentication, efficacy, safety and quality control. Samples were obtained from six different locations in Indonesia which included West Java (WJ), Central Java (CJ), East Java (EJ), East Borneo (EB), Central Borneo (CB), and South Borneo (SB). Metabolite fingerprinting was determined by HPTLC-densitometry method and profile of anticancer and toxicity were analyzed by MTT-ELISA method. The difference among metabolite fingerprinting, anticancer, and toxicity profile was analyzed by Principal Component Analysis (PCA) and Hierarchical Component Analysis (HCA), whereas the association among them was analyzed by Partial Least Square (PLS). The PCA results showed a difference in E. palmifolia metabolite fingerprints and the HCA results showed that six different regions were the same cluster. The PLS-DA analysis showed four significant metabolites proposed as anticancer markers with Rf 0.34, 0.59, 0.76, 0.93 and three significant metabolites proposed as negative markers with Rf 0.02, 0.44 and 0.59. E. palmifolia from East Java had the lowest IC50 (86.98±4.62µg/mL) and higher SI value (5.5).

Stres merupakan suatu keadaan tertekan baik secara fisik maupun secara psikologis. Suatu proses yang menilai suatu peristiwa sebagai sesuatu yg mengancam, ataupun membahayakan dan individu merespon peristiwa itu pada level fisiologis, emosional, kognitif, dan perilaku. Hormon kortisol adalah hormon stres, karena hormon ini akan diproduksi lebih banyak saat tubuh mengalami stres baik fisik maupun emosional. Terapi murottal Al-Qur'an merupakan salah satu terapi non farmakologis yang dapat digunakan untuk mempercepat proses penyembuhan. Lantunan Al-Qur'an secara fisik mengandung unsur suara manusia. Suara dapat menurunkan hormon stres, mengaktifkan hormon endorfin alami, meningkatkan perasaan rileks dan mengalihkan perhatian dari rasa takut, cemas, dan tegang, memperbaiki sistem kimia tubuh. Surat Ar-Rahman merupakan surat Makiyah yang mempunyai karakter ayat pendek dan berulang sehingga dapat menimbulkan efek relaksasi bagi pendengar. Tujuan dari penelitian ini adalah Menganalisis efektifitas pemberian terapi Murottal Al Qur'an terhadap kadar hormon kortisol pada mencit yang mengalami stres. Metode penelitian ini adalah true experimental post-test only control group design. 35 mencit dibagi menjadi 5 kelompok, Kelompok K+: tidak diberi perlakuan murottal QS. Ar-Rahman dan tidak diberi paparan stresor kebisingan; Kelompok Kontrol (-) diberi paparan stresor kebisingan 21 hari dan tidak diberi perlakuan murottal QS. Ar-Rahman; P1 diberi paparan stresor kebisingan 21 hari + murottal QS. Ar-Rahman 1 jam/hari; P2 diberi paparan stresor kebisingan 21 hari + murottal QS. Ar-Rahman 2 jam/hari; P3 diberi paparan stresor kebisingan 21 hari + murottal QS. Ar-Rahman 4 jam/hari. Analisis data menggunakan Median Test didapatkan nilai p value adalah 0,032 (p< 0,05) menyatakan terdapat perbedaan yang signifikan kadar kortisol pada mencit dengan kontrol negatif, kontrol positif dan yang mendapat perlakuan 1, 2, dan 3. Kesimpulan penelitian ini adalah bahwa Terapi Murottal Al Quran dapat menurunkan kadar kortisol mencit yang dipapar stres kebisingan.

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COVID-19 is a disease caused by SARS CoV-2 by producing structural proteins and non-structural proteins. SARS CoV-2 uses a spike glycoprotein to bind ACE-2 receptors in host cells and uses main protease to replicate. This research aims to screen green tea catechin derivates as an antiviral for SARS CoV-2 through inhibition of spike glycoprotein (6LZG) and main protease (5R7Y). In silico studies carried out are molecular docking, prediction of physicochemical properties, and prediction of toxicity. The potential inhibition was assessed based on binding affinity and interaction of amino acid residues. From the molecular docking process showed that epicatechin and epigallocatechin provide inhibition to spike glycoprotein better than nafamostat indicated by binding affinity of -5.2 kcal/mol and -4.5 kcal/mol, while epigallocatechin gallate and epicatechin gallate provide inhibiton to main protease better than lopinavir with binding affinity of -8.7 kcal/mol and -8.3 kcal/mol. The results of the physicochemical properties prediction showed that only epigallocatechin gallate that did not fulfill five Lipinski's rule. Based on the toxicity class LD50, the derivates of catechin belong to classes 4 and 6. In conclusion, it can be known that epicatechin and epigallocatechin can be developed as an antiviral for SARS CoV-2.