The aims of this study were to find out the potential of Patikan kerbau (Euphorbia hirta) as antibacterial on Aeromonas hydropilla and Vibrio alginolyticus. Phytochemical study on E. hirta leaves was carried out to determine the chemical compounds group and resulted in the presence of phenolic, terpenoid and tannin group in the leaves. The antibacterial assay by diffusion agar using paper disc showed that methanol and aqueou extract either of using dried powder leaves boiling method or fresh leaves boiling method were able to inhibit the growth of Aeromonas. hydropilla and Vibrio alginolyticus. The highest antibacterial activity against those bacteria was shown by methanol extract. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of this methanol extract was found to be 0.156 and 0.625 % by using agar dilution method. In addition, the toxicity study exhibited that treatment of cat fish (Clarias batrachus) larvae and humpback grouper (Cromileptes altivelis) larvae with E. hirta methanol extract at the concentration of 1 % for 5 minutes did not give any toxic effect.

This research was conducted for a month, in the laboratory of the Faculty of Marine and Fisheries of Nusa Cendana University. The study aims to determine the use of a banana kepok stem (Musa paradisiaca formmatypica) with different doses to see the type of natural feeds growing and the abundance of it. The banana kepok stem (Musa paradisiaca  formmatypica) generally contains nitrogen, phosphorus and potassium. Nitrogen and phosphorus important to growing of natural feeds, while potassium important to increase the body durability of it. This study uses different dosages to see the abundance of natural feed. The dose used is 50 g.L-1, 75 g.L-1, 100 g. L-1. The results of the study showed the type of natural feed that grows are Daphnia sp and Infusoria and the highest amount of natural feed density in the treatment with dose of 75 g that is Infusoria as much as 356x104 cells.ml-1 and Daphnia sp as much as 168x104 cells.ml-1. The use of stem banana kepok (Musa paradisiaca formmatypica) can be used as feed for fish. Keywords: Banana kepok  stem (Musa paradisiaca formmatypica),  Dosages, Natural feed

The Gracilaria genus is a potential source of natural and environmentally-friendly alternatives in improving the survival and growth of shrimp. This study aims to identification immunostimulant molecules extract G. verrucosa and evaluate the utilization of G verrucosa extract as an immunostimulant in improving survival and growth of L. vannamei. Seaweed extraction used ethyl acetate then formulated in the diets. The immunostimulant molecule in the G. verrucosa was analysis. The shrimp were fed a test diet containing extract G. verrucosa at a dose of 2 g kg-1 or extract G. verrucosa-free control diets for 42 days. Shrimps were fed diets containing extract with a specific duration. The observation on the survival and growth of L. vannamei was performed after maintenance at the Laboratory for six weeks. Following, diets containing extract was tested in the field (pond shrimp farm) at the same dose of extract for 58 days. Shrimp was feed diets containing extract once a week, once in the early culture, and diet control, then the survival and growth shrimp were analysis. Concentrations of sulfates and carbohydrates in G. verrucosa ethyl acetate-extract were 24.21% and 13.41%, and crude protein 3.64%. GC-MS pyrolysis results show that G. verrucosa polysaccharide is similar to immunostimulant molecules. The survival shrimp gave diets containing G. verrucosa extract formulation was higher than that of shrimps fed controls diet. The Shrimp fed diets extracts have higher growth than shrimp given control dietsKeywords: Gracilaria, extract, polysaccharides, immunostimulant

One of the parameters that play an important role in the success of vannamei shrimp cultivation is salinity. This study aims to determine the effect of decreasing salinity on the behavior and stress response of vannamei shrimp. The test animals used were vannamei shrimp, which were in logs with an average weight of 4.6 – 6.3g. This study used a completely randomized design (CRD) with different salinity reduction treatments in each maintenance medium, namely Treatment A without decreasing salinity, Treatment B decreasing salinity 2 ppt per 6 hours for 24 hours, Treatment C decreasing salinity 2 ppt per 4 hours for 24 hours, Treatment D decreased salinity 2 ppt per 2 hours for 24 hours, each treatment was repeated 3 times. Blood glucose was measured after the decrease in salinity and on the last day of the study. Blood glucose collection was carried out at the fifth swimming leg on white shrimp using a 1 ml syringe that had been rinsed using Na Citrate with the aim that the blood glucose taken did not clot quickly. The results showed a decrease in salinity caused by shrimp stress. The concentration of vannamei shrimp blood glucose increased (13.66 – 52.37mg/dl). Treatment D was the best glucose concentration to suppress blood glucose concentrations (13.66mg/dl). Based on the data analysis of blood glucose concentration (p<0.05) it continued with the real difference test. Vannamei shrimp behavior during salinity reduction showed a response that was not different from all treatments. Salinity 6 ppt is good salinity in the process of suppressing blood glucose concentrations when changes in water salinity occur.

Ikan Mas (Cyprinus carpio) adalah jenis ikan air tawar yang memiliki peluang dikembangakan karena memiliki potensi pasar tinggi serta permintaan yang terus meningkat. Banyak upaya telah dilakukan pembudidaya dalam memenuhi permintaan tersebut diantaranya melakukan budidaya menerapkan teknologi intensif. Namun kenyataannya, penerapan teknologi budidaya ini sering mengalami kendala seperti timbulnya penyakit yang diakibatkan oleh bakteri Aeromonas hydropilla. Tujuan dari penelitian ini untuk mengetahui pengaruh pemberian daun nangka (Artocarpus heterophyllus) terhadap infeksi bakteri Aeromonas hydrophila yang menyerang ikan mas (Cyprinus carpio). Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) 4 perlakuan 3 ulangan. Dosis daun nangka yang digunakan adalah perlakuan kontrol tanpa penggunaan daun nangka, perlakuan A daun nangka dengan dosis 20 ml, perlakuan B daun nangka 30 ml, perlakuan C daun nangka 40 ml. Hasil penelitian menunjukkan dosis pemberian daun nangka sebanyak 40 ml berpengaruh terhadap tingkat kesembuhan ikan mas (Cyprinus carpio) yang terinfeksi bakteri Aeromonas hydrophila