Potential of Patikan Kerbau (Euphorbia hirta) as Antibacterial on Aeromonas hydropilla and Vibrio alginolitycus in Fish Culture

Salosso, Yuliana; Jasmanindar, Yudiana

doi:10.5296/ast.v2i1.4916

Cited by 5 publications

(10 citation statements)

References 7 publications

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“…Water extraction was done by boiling 10 g of finely ground P. australis in 100 ml of distilled water (10%), and incubated for 24 h. The samples have then filtered and stored until further use. 14 Methanol extraction was done by macerating 10 g of finely ground P. australis in methanol for 48 h, filtered to separate the sediment and extract, then evaporated, and ready for the antibacterial test.…”

Section: P Australis Extractionmentioning

confidence: 99%

Nutrient Content, Active Compound and Antibacterial Activity of Padina australis against Aeromonas hydropilla

Salosso¹,

Aisiah²,

Toruan³

et al. 2020

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Macroalgae belong to thallophytes group or plant-like organisms which generally live in coastal areas. 1-3 Macroalgae known as seaweed have significant economic value since they can be utilized as vegetables, traditional medicines, organic fertilizers, and livestock fed. 1,3 Even the phytocoloid compounds extracted from macroalgae as agar, carrageenan, and alginate 3 , it can be used as raw materials of various industries, such as medicine, cosmetics, food, etc. Based on the pigment content, macroalgae are classified as green, red, and brown macroalgae. 4,5 Several studies has been revealed that green, brown and red algae contain different metabolites and have much biological activity such as antiviral, antibacterial and antifungal. 6 Padina australis is one of the brown macroalgae that possess numerous compounds that can be used for various properties, either nutrient content or active compounds. P. australis contains 1.05 ± 0.09% protein, 0.58 ± 0.01% fat, 8.78 ± 0.80% carbohydrate, 87.25 ± 0.86% water, 2.34 ± 0.16% ash 7 and minerals as calcium, magnesium, potassium, sodium, copper, zinc, iron 8 that enable to be developed as a food source and livestock fed. P. australis also contains various active compounds, such as steroid, terpenoid, flavonoid, tannin, and saponin 2,9,10 that can be used as medicinal drugs. P. australis found abundantly in Indonesia sea and distributed almost all stony coasts including East Nusa Tenggara waters. According to Salosso and Jasmanindar 11 , brown macroalgae recorded in 5 sampling sites of Kupang Bay and distributed in all locations and sampling sites are P. australis with up to 80% occurrence frequency. Nevertheless, this species has not been maximally utilized yet by the community. 10 To optimize the utilization of P. autralis collected from Kupang Bay, a study on nutrient content, active compounds, and antibacterial activity of P. australis were carried out in this paper. MATERIALS AND METHODS P. australis collection P. australis were collected at the lowest tide in Kelapa Lima coastal waters, Kupang Bay, by searching along the coast and taking all encountered P. australis. They were put into a plastic bag, cleansed, recorded the fresh weight, air-dried, and then ready for further analyses. Chemical composition analysis Chemical composition analyses of P. australis include water, ash, protein, and fat content. 12 Carbohydrate content was determined by difference as follows: 100%-(% water + % fat + % protein + % ash). Amino acid content was determined using a High-Performance Liquid Chromatography (HPLC). Mineral analyses on calcium (Ca), potassium (K), and iron (Fe) were assayed using Atomic Absorption Spectrophotometer (AAS). Phytochemical analysis of P. australis Phytochemical investigations of P. australis include alkaloid, saponin, flavonoid, tannin, terpenoid, and steroid. Alkaloid was examined using Culvenor-Fiztgerald method, saponin was analyzed using a foam test, tannin was analysed using FeCl, and terpenoid and steroid was analyzed using the Lieberman-Burchard meth...

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Section: P Australis Extractionmentioning

confidence: 99%

Nutrient Content, Active Compound and Antibacterial Activity of Padina australis against Aeromonas hydropilla

Salosso¹,

Aisiah²,

Toruan³

et al. 2020

Self Cite

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show abstract

“…They are also cheap, easily available and affordable [4]. In recent years, the natural product research has focused the work on isolation and identification of compounds for application mostly in pharmaceutical area [5] Many plants in different locations around the world have been discovered and estimated that there are about 2.5 million species of higher plants and the majority of these have not yet been examined for their pharmacological properties [6]. Plants have been used because of their antimicrobial properties, which are due to compounds synthesized in the secondary metabolism.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of methanolic root extracts of Euphorbia condylocarpa against pathogenic bacteria

Mohammadi¹,

Asgary²,

Shandiz³

et al. 2015

asb

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In the present study, the antibacterial activity effect of methanolic root extracts of Euphorbia Condylocarpa was evaluated against five Gram positive, three Gram negative bacterial strains. The antibacterial activity was determined by agar disk diffusion method. The most susceptible bacteria to Euphorbia Condylocarpa root extracts were Bacillus subtillis, B. pumilis and Staphylococcus epidermidis and displayed larger inhibition zones than Gram negative bacteria. Minimum inhibition concentrations (MIC) of extract against these bacteria strains were determined. The lowest MIC values were obtained with B. pumilis, B. subtillis and S. epidermidis (1.87 mg/mL), followed by Staphylococcus aureus and Escherichia coli (7.50 mg/mL) and the highest were obtained with Pseudomonas aeruginosa, Klebsiella pneumoniae and Enterococcus faecalis (15.00 mg/mL). The cell proliferation and standard cytotoxicity assays of Euphorbia Condylocarpa root extract were revealed that there is no toxic against L929 normal cell line. Based on the current investigation, it is potentially suggested that methanolic Euphorbia Condylocarpa root extract can be proposed as an antibacterial agent and it can be further assessed to discover bioactive natural products involved in its activity.

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“…Leaves of C. odorata were cleaned and dried by using herbs dryer at temperature below 40°C. Fifty hundred gram of dried leaves was ground into powder form by grinder as described by Salosso and Jusmanindar [24] and repeatedly extracted with methanol at room temperature till the residue was colourless. The methanol extracts were filtered through Whatman No.…”

Section: Extraction Of C Odoratamentioning

confidence: 99%

Potential Study of Kopasanda (Chromolaena odorata L.) Leaves as Antibacterial Against Vibrio harveyi, Disease Causative Agent of Tiger Shrimp (Penaeus monodon Fabricius) Post Larvae

Prajitno¹,

Nursyam²

2015

J Aquac Res Development

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Vibriosis caused by Vibrio harveyi is still being a serious bacterial disease problem in the black tiger shrimp Penaeus monodon aquaculture. Due to this disease, the mortality of tiger shrimp post larvae can reach 100%. Attempt has been made for this bacterial disease prevention by using mangrove associates secondary metabolites. This study aims to determine potential of kopasanda (C. odorata L.) leaves as natural antibacterial against Vibrio harveyi. Potential study was carried out by determinination of the best solvent used to extract the bioactive secondary metabolites of kopasanda leaves, determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Toxicity study was also done by using soaking method to determine the save concentrations of C. odorata leaves on tiger shrimp post larvae. C. odorata leaves was extracted with methanol to extract all the active components, then, partitioned into n-hexane, ethyl acetate, methanol and aqueous extract. The highest antibacterial activity against V. harveyi was shown by methanol extract. The results suggest that C. odorata leaves have a potential to be developed as a raw material source for vibriosis prevention. It is due to its strong anti-vibrio activity showed by the low MIC and MBC of 0.625 and 1.250 mg/ml, respectively. Furthermore, the methanol extract did not show any toxicon tiger shrimp (P. monodon) post larvae up to 2.500 mg/ml.

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Potential of Patikan Kerbau (Euphorbia hirta) as Antibacterial on Aeromonas hydropilla and Vibrio alginolitycus in Fish Culture

Cited by 5 publications

References 7 publications

Nutrient Content, Active Compound and Antibacterial Activity of Padina australis against Aeromonas hydropilla

Nutrient Content, Active Compound and Antibacterial Activity of Padina australis against Aeromonas hydropilla

Antimicrobial activity of methanolic root extracts of Euphorbia condylocarpa against pathogenic bacteria

Potential Study of Kopasanda (Chromolaena odorata L.) Leaves as Antibacterial Against Vibrio harveyi, Disease Causative Agent of Tiger Shrimp (Penaeus monodon Fabricius) Post Larvae

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