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Two kinds of a-glucosidase (I and II) were isolated from honey bees by salting-out chroma tography with ammonium sulfate. a-Glucosidase I was purified by chromatography on CMcellulose and gel filtration on. Sephadex G-100. a-Glucosidase II was purified by chroma tographies on DEAE-and CM-cellulose and by gel filtration on Bio-Gel P-150. Both enzyme not. Recently Huber and Mathison7,8) purified for the first time two kinds of sucrase* (a-glucosidase) from honey bees to homogeneity. They suggested that one of the sucrases might be the same as the invertase found in honey. We also tried to purify a-glucosidase from honey bees, and successfully isolated two kinds of a-glucosidase as a homogeneous protein by the purification procedures independent from those of Huber and Mathison.7,8) a-Glucosidases are known to distribute in various insects.9•`13) Recently it has been

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