Potato (Solanum tuberosum L.) is one of the five basic sources of carbohydrates. It consumed by many people world spreads. One of the constraints in potato production is the presence of fungal diseases. Fungi that cause diseases in potato crops include Phytophthora infestans which causes late blight, Fusarium oxysporum which cause fusarium wilt, Alternaria solani Sor. which cause brown spot disease, and Aspergillus niger which infect bulbs and produce aflatoxin. Identification of potatoes-contaminating fungi can lead the farmers to get a better potatoes production. This research goal is to identify potatoes-contaminating fungi in traditional market of Condong Catur, District of Sleman, Yogyakarta. This research was using descriptive method with laboratory examination. We took 30 defected potatoes suspected of being infected by fungi. Samples were taken from each potato aseptically and then cultured in Saboraud's Dextrose Agar (SDA) media. Observation was held for the fungi growth after 24 hours incubation. From the laboratory examination, there were fungal infections in all of potatoes and identified as Phytopthora infestans (26.67%), Fusarium oxysporum (86.67%), Alternaria solani Sor. (6.67%), and Aspergillus niger (13.33%). Phytophthora infestans, Fusarium oxysporum, Alternaria solani Sor., and Aspergillus niger identified as potatoescontaminating fungi in Traditional Market of Condong Catur, District of Sleman, Yogyakarta.

Background :. Escherichia coli is a bacteria cause of infections such as diarhea and urinary tractus infection. Antibiotic used for treatment of diarhea and urinary tractus infection include ampicilin, penicilin G, amoxicilin, chloramphenicol, tetracycline, and sulbenicin. These antibiotic are often used, but it has some side effects as well as the emergence of resistance. Treatment with minimal effects and does not cause mildew resistance continue to alternative antibacteria. Cabbage leaf is a vegetable which contains flavonoid, saponin, polifenol, and tanin that are antibacteria. The purpose of this study was to determine antibacteria effectivity ethanol extract of cabbage leaf (Brassica oleracea var.capitata f.alba) againts Escherichia coli bacteria in vitro. Method : This research is an experimental study with the method of diffusion (Kirby Bauer). Escherichia coli bacteria isolated obtained from Medical Laboratory Technology of Poltekkes Kemenkes Yogyakarta. Ethanol extract obtained through maseration extraction method and the concentration used were 60%, 70%, 80%, 80%, 90%, and 100%. Analysis of the research results in table and narration. Results : The results of inhibition zone is formed at a concentration of 60%, 70%, 80%, 90%, and 100% respectively are 11,33 mm, 12,33 mm, 13 mm, 14,33 mm, and 15 mm.). Conclusion : Ethanol extract of cabbage leaf (Brassica oleracea var.capitata f.alba) are antibacteria againts Escherichia coli bacteria with strong category in (10-20 mm).

Pendahuluan: Kearifan lokal, pengobatan tradisonal, dan pengetahuan etnobotani perlu dipelajari dan dikembangkan. Salah satu masalah yang masih sering terjadi pada masyarakat adalah munculnya penyakit demam berdarah yang disebabkan nyamuk Aedes Aegypti. Demam Berdarah Dengue merupakan penyakit yang ditakuti karena menurunkan konsentrasi trombosit ke tingkat rendah (trombositopenia) dan dapat menyebabkan perdarahan. Jumlah sel trombosit yang rendah, harus segera ditingkatkan untuk menghindari terjadinya syok. Secara empiris, masyarakat menggunakan air rebusan daun ubi jalar pada kasus-kasus DBD (Demam Berdarah Dengue) dan menunjukkan perbaikan. Penelitian ini bertujuan untuk mengetahui potensi etnomedicine daun ubi jalar ungu (ipomoea batatas l. poir) sebagai obat demam berdarah di wilayah Sleman, DIY. Selain itu juga untuk mengetahui persentase peningkatan jumlah trombosit pada Tikus Putih (Rattus norvegicus) dengan pemberian infusa Daun Ubi Jalar Ungu dan ubi jalar putih (Ipomoea batatas .L) Jenis penelitian: True experimental dengan rancangan penelitian pretest dan post test with control group design. Penelitian ini menggunakan 35 tikus putih jantan yang dibagi dalam 7 kelompok perlakuan infusa daun ubi jalar ungu yaitu kelompok kontrol, kelompok dosis ringan, kelompok dosis sedang dan kelompok dosis berat untuk masing-masing infusa daun ubi jalar. Tikus diturunkan jumlah trombositnya melalui mekanisme kerusakan limpa dengan induksi anilin secara intravena. Setelah 24 jam penginduksian, hewan coba diberi perlakuan sesuai kelompok. Semua kelompok dihitung jumlah trombositnya sebelum dan sesudah perlakuan. Data yang diperoleh dari hasil penelitian dianalisis secara deskriptif yang disajikan dalam bentuk tabel dan narasi.Hasil penelitian: Terjadi peningkatan jumlah trombosit pada kelompok kontrol sebanyak 43%. Pada kelompok infusa daun ubi jalar ungu dosis ringan tidak ada peningkatan, kelompok dosis sedang meningkat sebesar 94%, dan kelompok dosis tinggi diperoleh peningkatan sebesar 224%. Pada kelompok infusa dun ubi jalar putih terdapat peningkatan pada dosis tinggi yaitu 7,2 ml/200gr BB atau 26,46 %. Hasil uji statistik menunjukkan bahwa pemberian infusa daun ubi jalar putih pada pre test dan post test memiliki perbedaan yang bermakna dengan nilai p value 0,023. Sedangkan pemberian infusa daun ubi jalar ungu sebelum dan sesudah perlakuan tidak memiliki perbedaan secara bermakna yang ditunjukkan dengan nilai p value 0,550. Kesimpulan: Infusa daun ubi jalar ungu mampu meningkatkan jumlah trombosit yang lebih banyak dibandingkan dengan infusa daun ubi jalar putih. Infusa daun ubi jalar ungu dan ubi jalar putih memiliki potensi etnomedicine sebagai obat demam berdarah di Sleman, DIY. Kata Kunci: Ipomoea batatas, etnomedicine, tikus putih, Trombosit, Demam Berdarah J o u r n a l o f H e a l t h | 7 journal.gunabangsa.ac.id

Widal examination is a serological method to diagnose typhoid fever used by Godean II Public Health Center, Sleman Regency, Yogyakarta Special Region (DIY). However, this method is difficult to hold onto because there is no agreement on agglutination standards. A positive Widal test result is not necessarily typhoid fever. Therefore, the results really need to be confirmed by other supporting examinations. IgM anti Salmonella IgG rapid test is one of the serological tests to support the diagnosis of typhoid fever which has better sensitivity and specificity to handle specific antibodies against Salmonella typhi in serum. This study aims to examine the results of the serological test of Immunoglobulin Miu (IgM) Anti Salmonella gamma immunoglobulin (IgG) on positive widal samples at Godean II Public Health Center and to see the lowest positive widal titers IgM antisalmonella in the sample. This research is a descriptive study with accidental sampling technique. The method of examination used a rapid test immunochromatography to examine IgM IgG anti Salmonella typhi on positive widal serum samples at Puskesmas Godean II, Sleman, DIY. The results showed that 21.7% of the samples were positive IgG negative IgM; 8.7% of IgM samples were positive IgG positive, and 69.6% IgM negative IgG negative. Anti Salmonella IgM in widal positive samples appeared at titer 320. Anti Salmonella typhi IgM and IgG were detected in positive widal samples at Godean II Public Health Center. Immunoglobulin Miu (IgM) appears at titer 320.

The tomato sauce is a complementary food that is often served with other foods, such as chicken noodles. Various factors can be the cause of contamination of tomato sauce by microbes like bacteria. These factors include environmental conditions that are not clean or container that was left open. Bacterial contamination in tomato sauce can result not suitable for consumption. Given by the high public interest in the consumption of tomato sauce with chicken noodle, it is necessary to observe the bacterial contamination of chicken noodle tomato sauce. To determine the bacterial contamination in tomato sauce of chicken noodle in traditional market of Condong Catur, Sleman, Yogyakarta. The samples used were tomato sauces that are used by chicken noodle stalls in the Condong Catur traditional market. Sampling was also carried out by recording the environmental conditions stalls and tomato sauce serving. To determine the bacterial contamination, samples examined in the laboratory based on the total bacterial count method using plate count agar (PCA) media. Seven of the ten samples showed the total number of bacteria <10 4 CFU/ml, whereas the other three samples are not suitable for consumption by the total number of bacteria >10 4 CFU / ml. The main factors of high contamination is poor hygiene of chicken noodle stall environmental, tomato sauce containers that are less clean and are often left open.