Increasing numbers of explanatory variables tend to result in information redundancy and “dimensional disaster” in the quantitative remote sensing of forest aboveground biomass (AGB). Feature selection of model factors is an effective method for improving the accuracy of AGB estimates. Machine learning algorithms are also widely used in AGB estimation, although little research has addressed the use of the categorical boosting algorithm (CatBoost) for AGB estimation. Both feature selection and regression for AGB estimation models are typically performed with the same machine learning algorithm, but there is no evidence to suggest that this is the best method. Therefore, the present study focuses on evaluating the performance of the CatBoost algorithm for AGB estimation and comparing the performance of different combinations of feature selection methods and machine learning algorithms. AGB estimation models of four forest types were developed based on Landsat OLI data using three feature selection methods (recursive feature elimination (RFE), variable selection using random forests (VSURF), and least absolute shrinkage and selection operator (LASSO)) and three machine learning algorithms (random forest regression (RFR), extreme gradient boosting (XGBoost), and categorical boosting (CatBoost)). Feature selection had a significant influence on AGB estimation. RFE preserved the most informative features for AGB estimation and was superior to VSURF and LASSO. In addition, CatBoost improved the accuracy of the AGB estimation models compared with RFR and XGBoost. AGB estimation models using RFE for feature selection and CatBoost as the regression algorithm achieved the highest accuracy, with root mean square errors (RMSEs) of 26.54 Mg/ha for coniferous forest, 24.67 Mg/ha for broad-leaved forest, 22.62 Mg/ha for mixed forests, and 25.77 Mg/ha for all forests. The combination of RFE and CatBoost had better performance than the VSURF–RFR combination in which random forests were used for both feature selection and regression, indicating that feature selection and regression performed by a single machine learning algorithm may not always ensure optimal AGB estimation. It is promising to extending the application of new machine learning algorithms and feature selection methods to improve the accuracy of AGB estimates.

Pine wilt disease (PWD) is currently one of the main causes of large-scale forest destruction. To control the spread of PWD, it is essential to detect affected pine trees quickly. This study investigated the feasibility of using the object-oriented multi-scale segmentation algorithm to identify trees discolored by PWD. We used an unmanned aerial vehicle (UAV) platform equipped with an RGB digital camera to obtain high spatial resolution images, and multi-scale segmentation was applied to delineate the tree crown, coupling the use of object-oriented classification to classify trees discolored by PWD. Then, the optimal segmentation scale was implemented using the estimation of scale parameter (ESP2) plug-in. The feature space of the segmentation results was optimized, and appropriate features were selected for classification. The results showed that the optimal scale, shape, and compactness values of the tree crown segmentation algorithm were 56, 0.5, and 0.8, respectively. The producer’s accuracy (PA), user’s accuracy (UA), and F1 score were 0.722, 0.605, and 0.658, respectively. There were no significant classification errors in the final classification results, and the low accuracy was attributed to the low number of objects count caused by incorrect segmentation. The multi-scale segmentation and object-oriented classification method could accurately identify trees discolored by PWD with a straightforward and rapid processing. This study provides a technical method for monitoring the occurrence of PWD and identifying the discolored trees of disease using UAV-based high-resolution images.

Recently, ETHYLENE OVERPRODUCER 1 (ETO1) had been cloned and identified as a negative post-transcriptional regulator in the ethylene biosynthesis in Arabidopsis. However, little was known about the role of ETO1 in other species, especially in tomato, which was an ideal model for studying the biosynthesis of ethylene during tomato fruit ripening. In this study, a tomato ETHYLENE OVERPRODUCER 1-LIKE 1 (LeEOL1) was cloned. The LeEOL1 cDNA was 3,515 bp long and carried an ORF that putatively encoded a polypeptide of 886 amino acids with a predicted molecular mass of 95 kDa. It shared 74% identity in amino acid sequence with Arabidopsis EOL1 and had one BTB (Broad-complex, Tramtrack, Bric-à-brac) domain and two TPR (tetratricopeptide repeat) domains, which were also conserved domains in AtEOL1. RT-PCR analysis of the temporal expression of LeEOL1 showed that its transcript decreased companied with increase of ethylene production in tomato ripening. The level of LeEOL1 transcripts in wild type tomato fruit at mature green stage did not distinctively change when treated with exogenous ethylene.