The purpose. To analyze results on epizootic/epidemic supervision of staphylococcal infection contaminations in Ukraine and the world. Methods. Retrospective analysis of accessible references concerning spread of staphylococcus and diseases caused by them is carried out. Informational resources of international organizations (WHO; FAO; UN, European center for supervision and warning of diseases (ECDC), European department on safety of foodstuff (EFSA), special literature, etc.) allocated on Internet sources are studied. Results. Global problem is illuminated of circulation of staphylococcus among animals and people, contamination by them of plants of environment, in particular water, food stuffs of animal and vegetable origin, etc. Necessity is shown of detection of Methicillinum-resistant clones of staphylococcus among productive animals, animals-partners and people, and also urgency of reinforced monitoring over contamination of animal production, etc. Conclusions. Necessity is determined of implementation of the system of target monitoring over staphylococcus, in particular Methicillinum-resistant, as a link in circuit of measures aimed at prophylaxis of diseases, caused by microorganisms of that family.

ntroduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia coli, which developed resistance to antibacterial drugs.Material and methods. Raw milk samples were obtained from clinically healthy cows on farms from Kiev and Poltava regions to identify E . coli, Staphylococcus spp., Enterococcus spp. isolates. Antimicrobial susceptibility testing was performed using the EUCAST disk diffusion method and MU on “Determination of microbial susceptibility to antibacterial drugs”. Results. The examined milk samples revealed the presence of E . coli, Staphylococcus spp. and Enterococcus spp. isolates, which proves poor sanitary and hygienic conditions of milk production process. Escherichia coli isolates were found susceptible to Ampicillin/sulbactam, Cefoxitin (100%), Meropenem, Tobramycin (100%), Netilin, Tigecycline, Nitroxoline (100%), Gatifloxacin, and Nitrofurantoin (100%). The studied E. coli isolates were found resistant to Ampicillin (100%), Imipenem, Tetracycline, and Doxycycline (100%). 41.7% of isolates of Staphylococcus epidermidis, Staphylococcus aureus were found resistant to Oxacillin, of which 90% were resistant to Benzylpenicillin and 20% to Rifampicin. Conclusions.The circulation of antibiotic-resistant Enterobacteriaceae strains among farm animals is a major problem requiring a strategy development aimed to prevent the emergence and spread of antibiotic resistance worldwide.

Dogs and cats that live near humans are a source of zoono c agents. The purpose of the study was to inves gate domes c dogs and cats for staphylococcus carriers and to study their biological proper es, biofilm ability, and an bio c resistance in isolated cultures. A total of 44 samples were collected from 19 dogs and 25 cats of different age and sex groups. Staphylococci were detected in 54% of all samples selected. 25 cultures of Staphylococcus spp. were isolated. Plasma coagula on reac on was nega ve in 100% of strains isolated from cats and posi ve in 2 strains isolated from dogs (18%). Lecithinase ac vity was detected in 85.8% of strains isolated from cats and 72.8% from dogs. 71.5% of strains isolated from cats and 63.7% from dogs had the ability to induce hemolysis. Mannitol was fermented in 50.0% of strains isolated from cats and 54.6% from dogs. 78.6% of strains isolated from cats and 91.0% isolated from dogs grew on crystal violet lactose agar in the

Cow mastitis is a polyetiological disease involving pathogenic microorganisms. Antibiotics are used to treat sick animals, which contributes to the development of resistant clones of bacteria, and consumption of the corresponding milk leads to the spread of antibiotic-resistant microorganisms. The purpose of the work is to determine strains of antibiotic-resistant bacteria in the milk of cows suffering from subclinical mastitis. Bacteriological studies of mammary gland secretion samples were performed by inoculating the sediment obtained after centrifugation of the examined material on an agarised nutrient medium (with 5% ram erythrocytes). The study of antimicrobial sensitivity was performed by the disk diffusion method. It was established that pathogens with multiple resistance to antibiotics circulate among cows suffering from mastitis on the farm, which indicates their indiscriminate use in animal therapy schemes, in particular mastitis. It was found that the overall level of phenotypic resistance to antimicrobial agents of the examined isolates was within 19-33% (in Staphylococcus spp. – 19.9%, in E. coli – 33.2%, in Enterococcus spp. – 32.5%). The analysis of the results allowed assigning the culture study to the category of multiresistant: 31.6% of cultures of Staphylococcus spp. demonstrated resistance to five groups of antibiotics; 54.5% of Escherichia cultures – up to five groups of antibiotics; 37.5% of cultures of Enterococcus spp. resistant to three groups of antibiotics. It was established that 100% of the studied cultures of Staphylococcus spp. are sensitive to netilmicin, sparfloxacin, gatifloxacin and clarithromycin. Therewith, 100% of the examined cultures of Enterococcus spp. sensitive to gentamicin, norfloxacin, and nitrofurantoin, and 100% of the examined E. coli cultures were sensitive to cephalothin, meropenem, gentamicin, tobramycin, amikacin, nitroxoline, and gatifloxacin. Thus, dairy cows suffering from subclinical mastitis are a source of bacteria with multiple resistance to antibiotics and carry risks for consumers due to the consumption of dairy products contaminated with pathogens of zoonotic diseases, which requires proper control by veterinary service specialists

Staphylococci have a wide range of pathogenic properties, among which stands increased resistance to antibiotics and the ability to form a biofilm. Being divided into coagulase-positive and coagulase-negative, they have different and, at the same time, common biological properties and may be a source of genetic material for each other. The aim of the study was to examine the properties of staphylococci, isolated from various objects (milk, pigs, companion animals, humans), their resistance to antibiotics, the ability to form a biofilm and the presence of genes, responsible for resistance to methicillin and biofilm formation. In this study we used 89 Staphylococci strains. 18 (20.2 %) strains were coagulase-positive, where 2 (2.2 %) strains, isolated from different animals and humans, had a complete set of phenotypic (plasma coagulation, biofilm formation, resistance to oxacillin and benzylpenicillin) and genotypic (mec A, fem B, ica A, ica D) signs of pathogenicity. Coagulase-negative staphylococci, isolated from various animals and humans, had resistance to oxacillin, benzylpenicillin and the ability to form biofilms, and also had the corresponding genes in their structure (mec A, ica AB, ica D) in 3.3 % of cases. Staphylococci, isolated from different animals and humans, were able to form a biofilm and had the appropriate set of genes (ica D, ica AB) in 5.6 % of cases.